GSM3526593: Microglia cells from Trem2 +/- animal 1040 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq

SRX5173871 GSM3526593 GSM3526593: Microglia cells from Trem2 +/- animal 1040 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181297 GSM3526593 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526593 GSM3526593 GEO Accession GSM3526593 SRX5173872 GSM3526594 GSM3526594: Non-microglia cells from Trem2 +/- animal 1040 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181298 GSM3526594 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526594 GSM3526594 GEO Accession GSM3526594 SRX5173873 GSM3526595 GSM3526595: Microglia cells from Trem2 -/- animal 1104 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181299 GSM3526595 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526595 GSM3526595 GEO Accession GSM3526595 SRX5173874 GSM3526596 GSM3526596: Non-microglia cells from Trem2 -/- animal 1104 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181300 GSM3526596 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526596 GSM3526596 GEO Accession GSM3526596 SRX5173875 GSM3526597 GSM3526597: Microglia cells from Trem2 +/- animal 1106 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181301 GSM3526597 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526597 GSM3526597 GEO Accession GSM3526597 SRX5173876 GSM3526598 GSM3526598: Non-microglia cells from Trem2 +/- animal 1106 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181302 GSM3526598 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526598 GSM3526598 GEO Accession GSM3526598 SRX5173877 GSM3526599 GSM3526599: Microglia cells from Trem2 +/- animal 1108 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181303 GSM3526599 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526599 GSM3526599 GEO Accession GSM3526599 SRX5173878 GSM3526600 GSM3526600: Non-microglia cells from Trem2 +/- animal 1108 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181304 GSM3526600 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526600 GSM3526600 GEO Accession GSM3526600 SRX5173879 GSM3526601 GSM3526601: Microglia cells from Trem2 -/- animal 1744 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181305 GSM3526601 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526601 GSM3526601 GEO Accession GSM3526601 SRX5173880 GSM3526602 GSM3526602: Non-microglia cells from Trem2 -/- animal 1744 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181306 GSM3526602 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526602 GSM3526602 GEO Accession GSM3526602 SRX5173881 GSM3526603 GSM3526603: Microglia cells from Trem2 +/- animal 1745 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181307 GSM3526603 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526603 GSM3526603 GEO Accession GSM3526603 SRX5173882 GSM3526604 GSM3526604: Non-microglia cells from Trem2 +/- animal 1745 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181308 GSM3526604 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526604 GSM3526604 GEO Accession GSM3526604 SRX5173883 GSM3526605 GSM3526605: Microglia cells from Trem2 +/+ animal 1746 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181309 GSM3526605 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526605 GSM3526605 GEO Accession GSM3526605 SRX5173884 GSM3526606 GSM3526606: Non-microglia cells from Trem2 +/+ animal 1746 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181310 GSM3526606 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526606 GSM3526606 GEO Accession GSM3526606 SRX5173885 GSM3526607 GSM3526607: Microglia cells from Trem2 -/- animal 1748 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181311 GSM3526607 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526607 GSM3526607 GEO Accession GSM3526607 SRX5173886 GSM3526608 GSM3526608: Non-microglia cells from Trem2 -/- animal 1748 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181312 GSM3526608 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526608 GSM3526608 GEO Accession GSM3526608 SRX5173887 GSM3526609 GSM3526609: Microglia cells from Trem2 +/+ animal 1930 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181313 GSM3526609 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526609 GSM3526609 GEO Accession GSM3526609 SRX5173888 GSM3526610 GSM3526610: Non-microglia cells from Trem2 +/+ animal 1930 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181314 GSM3526610 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526610 GSM3526610 GEO Accession GSM3526610 SRX5173889 GSM3526611 GSM3526611: Microglia cells from Trem2 +/+ animal 1932 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181315 GSM3526611 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526611 GSM3526611 GEO Accession GSM3526611 SRX5173890 GSM3526612 GSM3526612: Non-microglia cells from Trem2 +/+ animal 1932 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181316 GSM3526612 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526612 GSM3526612 GEO Accession GSM3526612 SRX5173891 GSM3526613 GSM3526613: Microglia cells from Trem2 +/+ animal 2474 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181317 GSM3526613 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526613 GSM3526613 GEO Accession GSM3526613 SRX5173892 GSM3526614 GSM3526614: Non-microglia cells from Trem2 +/+ animal 2474 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181318 GSM3526614 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526614 GSM3526614 GEO Accession GSM3526614 SRX5173893 GSM3526615 GSM3526615: Microglia cells from Trem2 +/- animal 2476 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181319 GSM3526615 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526615 GSM3526615 GEO Accession GSM3526615 SRX5173894 GSM3526616 GSM3526616: Non-microglia cells from Trem2 +/- animal 2476 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181320 GSM3526616 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526616 GSM3526616 GEO Accession GSM3526616 SRX5173895 GSM3526617 GSM3526617: Microglia cells from Trem2 -/- animal 2478 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181321 GSM3526617 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526617 GSM3526617 GEO Accession GSM3526617 SRX5173896 GSM3526618 GSM3526618: Non-microglia cells from Trem2 -/- animal 2478 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181322 GSM3526618 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526618 GSM3526618 GEO Accession GSM3526618 SRX5173897 GSM3526619 GSM3526619: Microglia cells from Trem2 +/+ animal 309 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181323 GSM3526619 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526619 GSM3526619 GEO Accession GSM3526619 SRX5173898 GSM3526620 GSM3526620: Non-microglia cells from Trem2 +/+ animal 309 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181324 GSM3526620 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526620 GSM3526620 GEO Accession GSM3526620 SRX5173899 GSM3526621 GSM3526621: Microglia cells from Trem2 -/- animal 310 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181325 GSM3526621 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526621 GSM3526621 GEO Accession GSM3526621 SRX5173900 GSM3526622 GSM3526622: Non-microglia cells from Trem2 -/- animal 310 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181326 GSM3526622 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526622 GSM3526622 GEO Accession GSM3526622 SRX5173901 GSM3526623 GSM3526623: Microglia cells from Trem2 +/+ animal 3917 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181327 GSM3526623 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526623 GSM3526623 GEO Accession GSM3526623 SRX5173902 GSM3526624 GSM3526624: Non-microglia cells from Trem2 +/+ animal 3917 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181328 GSM3526624 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526624 GSM3526624 GEO Accession GSM3526624 SRX5173903 GSM3526625 GSM3526625: Microglia cells from Trem2 +/- animal 4352 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181329 GSM3526625 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526625 GSM3526625 GEO Accession GSM3526625 SRX5173904 GSM3526626 GSM3526626: Non-microglia cells from Trem2 +/- animal 4352 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181330 GSM3526626 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526626 GSM3526626 GEO Accession GSM3526626 SRX5173905 GSM3526627 GSM3526627: Microglia cells from Trem2 +/+ animal 5512 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181331 GSM3526627 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526627 GSM3526627 GEO Accession GSM3526627 SRX5173906 GSM3526628 GSM3526628: Non-microglia cells from Trem2 +/+ animal 5512 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181332 GSM3526628 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526628 GSM3526628 GEO Accession GSM3526628 SRX5173907 GSM3526629 GSM3526629: Microglia cells from Trem2 +/- animal 6296 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181333 GSM3526629 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526629 GSM3526629 GEO Accession GSM3526629 SRX5173908 GSM3526630 GSM3526630: Non-microglia cells from Trem2 +/- animal 6296 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181334 GSM3526630 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526630 GSM3526630 GEO Accession GSM3526630 SRX5173909 GSM3526631 GSM3526631: Microglia cells from Trem2 +/+ animal 743 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181335 GSM3526631 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526631 GSM3526631 GEO Accession GSM3526631 SRX5173910 GSM3526632 GSM3526632: Non-microglia cells from Trem2 +/+ animal 743 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181336 GSM3526632 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526632 GSM3526632 GEO Accession GSM3526632 SRX5173911 GSM3526633 GSM3526633: Microglia cells from Trem2 +/- animal 7674 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181337 GSM3526633 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526633 GSM3526633 GEO Accession GSM3526633 SRX5173912 GSM3526634 GSM3526634: Non-microglia cells from Trem2 +/- animal 7674 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181338 GSM3526634 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526634 GSM3526634 GEO Accession GSM3526634 SRX5173913 GSM3526635 GSM3526635: Microglia cells from Trem2 +/- animal 8042 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181339 GSM3526635 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526635 GSM3526635 GEO Accession GSM3526635 SRX5173914 GSM3526636 GSM3526636: Non-microglia cells from Trem2 +/- animal 8042 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181340 GSM3526636 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526636 GSM3526636 GEO Accession GSM3526636 SRX5173915 GSM3526637 GSM3526637: Microglia cells from Trem2 +/+ animal 8043 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181341 GSM3526637 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526637 GSM3526637 GEO Accession GSM3526637 SRX5173916 GSM3526638 GSM3526638: Non-microglia cells from Trem2 +/+ animal 8043 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181342 GSM3526638 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526638 GSM3526638 GEO Accession GSM3526638 SRX5173917 GSM3526639 GSM3526639: Microglia cells from Trem2 -/- animal 8485 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181343 GSM3526639 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526639 GSM3526639 GEO Accession GSM3526639 SRX5173918 GSM3526640 GSM3526640: Non-microglia cells from Trem2 -/- animal 8485 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181344 GSM3526640 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526640 GSM3526640 GEO Accession GSM3526640 SRX5173919 GSM3526641 GSM3526641: Microglia cells from Trem2 -/- animal 8635 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181345 GSM3526641 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526641 GSM3526641 GEO Accession GSM3526641 SRX5173920 GSM3526642 GSM3526642: Non-microglia cells from Trem2 -/- animal 8635 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181346 GSM3526642 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526642 GSM3526642 GEO Accession GSM3526642 SRX5173921 GSM3526643 GSM3526643: Microglia cells from Trem2 +/+ animal 9144 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181347 GSM3526643 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526643 GSM3526643 GEO Accession GSM3526643 SRX5173922 GSM3526644 GSM3526644: Non-microglia cells from Trem2 +/+ animal 9144 after 5 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181348 GSM3526644 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526644 GSM3526644 GEO Accession GSM3526644 SRX5173923 GSM3526645 GSM3526645: Microglia cells from Trem2 +/+ animal 9145 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181349 GSM3526645 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526645 GSM3526645 GEO Accession GSM3526645 SRX5173924 GSM3526646 GSM3526646: Non-microglia cells from Trem2 +/+ animal 9145 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181350 GSM3526646 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526646 GSM3526646 GEO Accession GSM3526646 SRX5173925 GSM3526647 GSM3526647: Microglia cells from Trem2 +/- animal 943 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181351 GSM3526647 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526647 GSM3526647 GEO Accession GSM3526647 SRX5173926 GSM3526648 GSM3526648: Non-microglia cells from Trem2 +/- animal 943 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181352 GSM3526648 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526648 GSM3526648 GEO Accession GSM3526648 SRX5173927 GSM3526649 GSM3526649: Microglia cells from Trem2 +/- animal 944 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181353 GSM3526649 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526649 GSM3526649 GEO Accession GSM3526649 SRX5173928 GSM3526650 GSM3526650: Non-microglia cells from Trem2 +/- animal 944 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181354 GSM3526650 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526650 GSM3526650 GEO Accession GSM3526650 SRX5173929 GSM3526651 GSM3526651: Microglia cells from Trem2 -/- animal 945 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181355 GSM3526651 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526651 GSM3526651 GEO Accession GSM3526651 SRX5173930 GSM3526652 GSM3526652: Non-microglia cells from Trem2 -/- animal 945 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181356 GSM3526652 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526652 GSM3526652 GEO Accession GSM3526652 SRX5173931 GSM3526653 GSM3526653: Microglia cells from Trem2 +/- animal 9463 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181357 GSM3526653 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526653 GSM3526653 GEO Accession GSM3526653 SRX5173932 GSM3526654 GSM3526654: Non-microglia cells from Trem2 +/- animal 9463 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181358 GSM3526654 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526654 GSM3526654 GEO Accession GSM3526654 SRX5173933 GSM3526655 GSM3526655: Microglia cells from Trem2 -/- animal 9467 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181359 GSM3526655 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526655 GSM3526655 GEO Accession GSM3526655 SRX5173934 GSM3526656 GSM3526656: Non-microglia cells from Trem2 -/- animal 9467 after 12 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181360 GSM3526656 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526656 GSM3526656 GEO Accession GSM3526656 SRX5173935 GSM3526657 GSM3526657: Microglia cells from Trem2 +/- animal 9469 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181361 GSM3526657 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526657 GSM3526657 GEO Accession GSM3526657 SRX5173936 GSM3526658 GSM3526658: Non-microglia cells from Trem2 +/- animal 9469 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181362 GSM3526658 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526658 GSM3526658 GEO Accession GSM3526658 SRX5173937 GSM3526659 GSM3526659: Microglia cells from Trem2 -/- animal 9470 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181363 GSM3526659 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526659 GSM3526659 GEO Accession GSM3526659 SRX5173938 GSM3526660 GSM3526660: Non-microglia cells from Trem2 -/- animal 9470 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181364 GSM3526660 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526660 GSM3526660 GEO Accession GSM3526660 SRX5173939 GSM3526661 GSM3526661: Microglia cells from Trem2 -/- animal 9542 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181365 GSM3526661 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526661 GSM3526661 GEO Accession GSM3526661 SRX5173940 GSM3526662 GSM3526662: Non-microglia cells from Trem2 -/- animal 9542 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181366 GSM3526662 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526662 GSM3526662 GEO Accession GSM3526662 SRX5173941 GSM3526663 GSM3526663: Microglia cells from Trem2 +/- animal 9546 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181367 GSM3526663 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526663 GSM3526663 GEO Accession GSM3526663 SRX5173942 GSM3526664 GSM3526664: Non-microglia cells from Trem2 +/- animal 9546 after 5 weeks on control diet; Mus musculus; RNA-Seq SRP174208 SRS4181368 GSM3526664 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526664 GSM3526664 GEO Accession GSM3526664 SRX5173943 GSM3526665 GSM3526665: Microglia cells from Trem2 -/- animal 981 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181369 GSM3526665 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526665 GSM3526665 GEO Accession GSM3526665 SRX5173944 GSM3526666 GSM3526666: Non-microglia cells from Trem2 -/- animal 981 after 12 weeks on cuprizone diet; Mus musculus; RNA-Seq SRP174208 SRS4181370 GSM3526666 RNA-Seq TRANSCRIPTOMIC cDNA Mice were perfused with PBS, brain were removed and dissociated with the Adult Brain Dissociation Kit (Miltenyi). Cells were fluorescence-activated cell sorted for viability, then microglia (Cd11b+) and all other cells were collected for RNA extaction using the RNeasy Plus Micro kit (Qiagen) Libraries were prepared using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), following the 'low-input' procedure defined by the manufacturer. Illumina HiSeq 4000 gds 303526666 GSM3526666 GEO Accession GSM3526666