SRX5190721 SD-Rye_1_2 SRP174908 bp0 10l of the normalized DNA from each accession was double-digested with PstI and MspI restriction enzymes then ligated to two adapters with barcode. After the adapters were ligated, the samples were pooled together for PCR amplification and subsequently size-selected for 250-300bp amplicons using the E-gel system . Libraries (80 pM) were prepared and sequenced in an Ion Proton System using Ion PI Hi-Q Chef and Sequencing 200 kits with two Ion P1v3 chips . SRS4196896 GBSRye_SD_02 SD-Rye_1_2 RAD-Seq GENOMIC Reduced Representation Ion Torrent Proton SRX5190722 SD-Rye_1_1 SRP174908 bp0 10l of the normalized DNA from each accession was double-digested with PstI and MspI restriction enzymes then ligated to two adapters with barcode. After the adapters were ligated, the samples were pooled together for PCR amplification and subsequently size-selected for 250-300bp amplicons using the E-gel system . Libraries (80 pM) were prepared and sequenced in an Ion Proton System using Ion PI Hi-Q Chef and Sequencing 200 kits with two Ion P1v3 chips . SRS4196897 RyeGBS_SD_01 SD-Rye_1_1 RAD-Seq GENOMIC Reduced Representation Ion Torrent Proton