SRX5210306 GSM3543717 SRP176107 SRS4214621 GSM3543717 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543717 GSM3543717 GEO Accession GSM3543717 SRX5210307 GSM3543718 SRP176107 SRS4214622 GSM3543718 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543718 GSM3543718 GEO Accession GSM3543718 SRX5210308 GSM3543719 SRP176107 SRS4214623 GSM3543719 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543719 GSM3543719 GEO Accession GSM3543719 SRX5210309 GSM3543720 SRP176107 SRS4214625 GSM3543720 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543720 GSM3543720 GEO Accession GSM3543720 SRX5210310 GSM3543721 SRP176107 SRS4214624 GSM3543721 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543721 GSM3543721 GEO Accession GSM3543721 SRX5210311 GSM3543722 SRP176107 SRS4214627 GSM3543722 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543722 GSM3543722 GEO Accession GSM3543722 SRX5210312 GSM3543723 SRP176107 SRS4214626 GSM3543723 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543723 GSM3543723 GEO Accession GSM3543723 SRX5210313 GSM3543724 SRP176107 SRS4214629 GSM3543724 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543724 GSM3543724 GEO Accession GSM3543724 SRX5210314 GSM3543725 SRP176107 SRS4214628 GSM3543725 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543725 GSM3543725 GEO Accession GSM3543725 SRX5210315 GSM3543726 SRP176107 SRS4214630 GSM3543726 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543726 GSM3543726 GEO Accession GSM3543726 SRX5210317 GSM3543727 SRP176107 SRS4214632 GSM3543727 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543727 GSM3543727 GEO Accession GSM3543727 SRX5210318 GSM3543728 SRP176107 SRS4214633 GSM3543728 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543728 GSM3543728 GEO Accession GSM3543728 SRX5210319 GSM3543729 SRP176107 SRS4214634 GSM3543729 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543729 GSM3543729 GEO Accession GSM3543729 SRX5210320 GSM3543730 SRP176107 SRS4214636 GSM3543730 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543730 GSM3543730 GEO Accession GSM3543730 SRX5210321 GSM3543731 SRP176107 SRS4214635 GSM3543731 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543731 GSM3543731 GEO Accession GSM3543731 SRX5210322 GSM3543732 SRP176107 SRS4214637 GSM3543732 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543732 GSM3543732 GEO Accession GSM3543732 SRX5210323 GSM3543733 SRP176107 SRS4214638 GSM3543733 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543733 GSM3543733 GEO Accession GSM3543733 SRX5210324 GSM3543734 SRP176107 SRS4214639 GSM3543734 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543734 GSM3543734 GEO Accession GSM3543734 SRX5210325 GSM3543735 SRP176107 SRS4214640 GSM3543735 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543735 GSM3543735 GEO Accession GSM3543735 SRX5210326 GSM3543736 SRP176107 SRS4214641 GSM3543736 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543736 GSM3543736 GEO Accession GSM3543736 SRX5210327 GSM3543737 SRP176107 SRS4214642 GSM3543737 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543737 GSM3543737 GEO Accession GSM3543737 SRX5210328 GSM3543738 SRP176107 SRS4214643 GSM3543738 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543738 GSM3543738 GEO Accession GSM3543738 SRX5210329 GSM3543739 SRP176107 SRS4214644 GSM3543739 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543739 GSM3543739 GEO Accession GSM3543739 SRX5210330 GSM3543740 SRP176107 SRS4214645 GSM3543740 Bisulfite-Seq GENOMIC Reduced Representation DNA extracted using the AllPrep DNA/RNA purification kit (QIAGEN) according to the manufacturer's instructions RRBS libraries were generated starting with genomic DNA extracted from WAT. Overnight digestion was performed on ~200ng DNA using the restriction enzyme MspI (New England Biolabs, Ipswich, MA). Reaction clean-up was performed with AMPure XP magnetic beads (Beckman Coulter, Brea, CA) before use with the NEXTflex Bisulfite-Seq Kit (Bioo Scientific Corporation, Austin, TX) for library preparation. The digested DNA was quantified and then ~80ng was end-repaired, A-tailed, and ligated with the NEBNext Methylated Adaptor (New England Biolabs). The ligated DNA was cleaned and then size-selected using AMPure XP magnetic beads to produce a final library size of 350 bp. Bisulfite conversion was performed with the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA) before carrying out PCR amplification with NEBNext Multiplex Oligos for Illumina (New England Biolabs) to barcode each library. Two AMPure XP bead cleans were performed and the resulting libraries were quantified with the Qubit High Sensitivity dsDNA Assay (Life Technologies, Eugene, OR) and the Bioanalyzer High Sensitivity DNA Kit (Agilent, Santa Clara, CA). NextSeq 500 gds 303543740 GSM3543740 GEO Accession GSM3543740