SRP176490 PRJNA513387 Transcriptome analysis in three development stage of seed among two Tartary Buckwheat cultivars reveals the mechanism of starch accumulation and biosynthesis Tartary buckwheat, the one of Fagopyrum species, which contained a large number of medical and nutritional components is becoming an important small grain crop. The Tartary buckwheat seed contains different of starches that influenced the quality of seed. To investigated the gene regulatory related starch accumulation at the different Tatary buckwheat cultivars and different stage of seed development, we performed a transcriptome analysis in two Tartary buckwheat cultivars using RNA-seq. Sequencing data generated approximately 95.61 Gb clean data. All of these data in each sample were aligned to the reference genome sequences of the Tartary buckwheat (Pinku1). And then, we obtained the expression pattern of these transcripts and assessed genes involved in starch metabolism. The DEGs between the two sample and the different seed development were detected. Based on their expression patterns, the DEGs were clustered, and the GO, KEGG annotation were analyzed. In addition, 48 DEGs in two samples related starch accumulation were identified. Furthermore, we predicted the novel genes related starch metabolism and identified the alternative splicing in Tartary buckwheat. Our findings provide gene expression profile and differential expression information in different cultivars of Tartary buckwheat.