SRP176648 PRJNA513834 16S rRNA sequences of Amazon soils over forest and agriculture use Soil microorganisms play important role in regulation of ecological processes, such as N cycle. Understanding the influence of anthropogenic activities, over soil microbial communities would increase our ability to establish conservation strategies, mainly in Amazon region, where deforestation has become the main agent of disturbance. In this study, we hypothesized that forest-to-agriculture conversion in a short- and long-term is responsible for alterations in soil chemical properties, which consequently alters the composition, diversity, and potential function of microbial communities. For this, we used molecular approaches as amplicon sequencing and quantification of marker genes to describe the microbial composition and abundance in soils of natural forest and agriculture fields with 2-, 8- and 20-years after conversion, in Amazon region.