SRP178958 PRJNA514964 pig gut metagenome Metagenome Dietary inclusion of resistant starches can promote host health through modulation of the gastrointestinal microbiota. In this study, we investigated This study elucidated the effect of transglycosylated starch (TGS) on the retention of digesta in the upper digestive tract, ileal flow and hindgut disappearance of nutrients, and bacterial microbiome profiles in ileal cannulated pigs. Fourteen ileal-cannulated growing pigs were fed either the TGS or control (CON) diet in a complete crossover design. Each period consisted of a 10-day adaptation to the diets, followed by 3-day collection of faeces and ileal digesta. For bacterial analysis, we collected fresh feces and ileal digesta from the cannula. Bacterial profiles of the pigs were determined using the Illumina MiSeq platform and sequencing of the V3-V5 region of the 16S rRNA gene. pig gut metagenome