SRX5250665 sictl_igf_1 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252312 sictl_igf_1 sictl_igf_1 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250666 sictl_ctl_3 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252313 sictl_ctl_3 sictl_ctl_3 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250667 sictl_ctl_2 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252314 sictl_ctl_2 sictl_ctl_2 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250668 sictl_ctl_1 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252315 sictl_ctl_1 sictl_ctl_1 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250669 sisnhg7_ctl_2 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252316 sisnhg7_ctl_2 sisnhg7_ctl_2 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250670 sisnhg7_ctl_1 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252317 sisnhg7_ctl_1 sisnhg7_ctl_1 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250671 sictl_igf_3 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252318 sictl_igf_3 sictl_igf_3 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250672 sictl_igf_2 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252319 sictl_igf_2 sictl_igf_2 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250673 sisnhg7_igf_1 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252320 sisnhg7_igf_1 sisnhg7_igf_1 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250674 sisnhg7_ctl_3 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252322 sisnhg7_ctl_3 sisnhg7_ctl_3 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250675 sisnhg7_igf_3 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252321 sisnhg7_igf_3 sisnhg7_igf_3 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500 SRX5250676 sisnhg7_igf_2 SRP179498 bp0 MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. SRS4252324 sisnhg7_igf_2 sisnhg7_igf_2 RNA-Seq TRANSCRIPTOMIC RT-PCR NextSeq 500