SRS4252312 SAMN10746879 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siCtl_pool_72hrs_IGF_8hrs biological_replicate 1 BioSampleModel Human SRS4252313 SAMN10746878 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siCtl_pool_72hrs_veh_8hrs biological_replicate 3 BioSampleModel Human SRS4252314 SAMN10746877 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siCtl_pool_72hrs_veh_8hrs biological_replicate 2 BioSampleModel Human SRS4252315 SAMN10746876 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siCtl_pool_72hrs_veh_8hrs biological_replicate 1 BioSampleModel Human SRS4252316 SAMN10746883 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siSNHG7_pool_72hrs_veh_8hrs biological_replicate 2 BioSampleModel Human SRS4252317 SAMN10746882 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siSNHG7_pool_72hrs_veh_8hrs biological_replicate 1 BioSampleModel Human SRS4252318 SAMN10746881 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siCtl_pool_72hrs_IGF_8hrs biological_replicate 3 BioSampleModel Human SRS4252319 SAMN10746880 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siCtl_pool_72hrs_IGF_8hrs biological_replicate 2 BioSampleModel Human SRS4252320 SAMN10746885 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siSNHG7_pool_72hrs_IGF_8hrs biological_replicate 1 BioSampleModel Human SRS4252321 SAMN10746887 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siSNHG7_pool_72hrs_IGF_8hrs biological_replicate 3 BioSampleModel Human SRS4252322 SAMN10746884 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siSNHG7_pool_72hrs_veh_8hrs biological_replicate 3 BioSampleModel Human SRS4252324 SAMN10746886 9606 Homo sapiens MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq. bioproject 515247 PRJNA515247 isolate cell line age less than passage 20 from ATCC biomaterial_provider ATCC sex female tissue mammary gland, breast; derived from metastatic site: pleural effusion cell_line MCF7 cell_subtype ER positive cell_type Epithelial dev_stage breast cancer disease adenocarcinoma race White sample_type cell culture treatment siSNHG7_pool_72hrs_IGF_8hrs biological_replicate 2 BioSampleModel Human