SRP180143 PRJNA515820 GSE125301 Inhibition of Phosphoinositide-3-kinase signaling promotes the stem cell state of trophoblast We found that PI3K inhibition increased the expression of stem cell markers in trophoblast stem cells (TSCs). To better understand the PI3K inhibited cells, we compared untreated TSCs with cells treated with PI3K inhibitor ZSTK474 for 3h, 6h and 3 days. Overall design: Untreated TSCs, TSCs treated with 200nM ZSTK474 for 3h, 6h, and 3 days. GSE125301 pubmed 31233251