SRX5267038 GSM3572760 SRP180361 SRS4270655 GSM3572760 RNA-Seq TRANSCRIPTOMIC cDNA Testicular single cells were encapsulated and barcoded with the inDrop microfluidics platform as instructed by the manufacturer (1CellBio). barcoded single-cell cDNA was purified with Agencourt RNAClean XP magnetic beads (Beckman Coulter, Cat. A63987) followed by second-strand synthesis reaction with NEBNext mRNA Second Strand Synthesis KIT (New England Biolabs, Cat. E6111S). Then linear amplification of cDNA was carried out through in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat. E2040S). IVT-amplified RNA was fragmented and purified again with Agencourt RNAClean XP magnetic beads. The second reverse transcription was done with PrimeScriptTM Reverse Transcriptase (Takara Clonetech, Cat. 2680A) followed with cDNA purification with Agencourt AMPure XP magnetic beads (Beckman Coulter, Cat.A63881). cDNA quantity was determined by qPCR on a fraction (5%) of purified cDNA. Final PCR amplification was done according to qPCR results and purified with Agencourt AMPure XP magnetic beads. Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen, Cat. Q32851). Library size was determined by Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat. 5067-4626). NextSeq 550 gds 303572760 GSM3572760 GEO Accession GSM3572760 SRX5267040 GSM3572761 SRP180361 SRS4270656 GSM3572761 RNA-Seq TRANSCRIPTOMIC cDNA Testicular single cells were encapsulated and barcoded with the inDrop microfluidics platform as instructed by the manufacturer (1CellBio). barcoded single-cell cDNA was purified with Agencourt RNAClean XP magnetic beads (Beckman Coulter, Cat. A63987) followed by second-strand synthesis reaction with NEBNext mRNA Second Strand Synthesis KIT (New England Biolabs, Cat. E6111S). Then linear amplification of cDNA was carried out through in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat. E2040S). IVT-amplified RNA was fragmented and purified again with Agencourt RNAClean XP magnetic beads. The second reverse transcription was done with PrimeScriptTM Reverse Transcriptase (Takara Clonetech, Cat. 2680A) followed with cDNA purification with Agencourt AMPure XP magnetic beads (Beckman Coulter, Cat.A63881). cDNA quantity was determined by qPCR on a fraction (5%) of purified cDNA. Final PCR amplification was done according to qPCR results and purified with Agencourt AMPure XP magnetic beads. Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen, Cat. Q32851). Library size was determined by Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat. 5067-4626). NextSeq 550 gds 303572761 GSM3572761 GEO Accession GSM3572761 SRX5267042 GSM3572762 SRP180361 SRS4270657 GSM3572762 RNA-Seq TRANSCRIPTOMIC cDNA Testicular single cells were encapsulated and barcoded with the inDrop microfluidics platform as instructed by the manufacturer (1CellBio). barcoded single-cell cDNA was purified with Agencourt RNAClean XP magnetic beads (Beckman Coulter, Cat. A63987) followed by second-strand synthesis reaction with NEBNext mRNA Second Strand Synthesis KIT (New England Biolabs, Cat. E6111S). Then linear amplification of cDNA was carried out through in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat. E2040S). IVT-amplified RNA was fragmented and purified again with Agencourt RNAClean XP magnetic beads. The second reverse transcription was done with PrimeScriptTM Reverse Transcriptase (Takara Clonetech, Cat. 2680A) followed with cDNA purification with Agencourt AMPure XP magnetic beads (Beckman Coulter, Cat.A63881). cDNA quantity was determined by qPCR on a fraction (5%) of purified cDNA. Final PCR amplification was done according to qPCR results and purified with Agencourt AMPure XP magnetic beads. Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen, Cat. Q32851). Library size was determined by Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat. 5067-4626). NextSeq 550 gds 303572762 GSM3572762 GEO Accession GSM3572762 SRX5267044 GSM3572763 SRP180361 SRS4270658 GSM3572763 RNA-Seq TRANSCRIPTOMIC cDNA Testicular single cells were encapsulated and barcoded with the inDrop microfluidics platform as instructed by the manufacturer (1CellBio). barcoded single-cell cDNA was purified with Agencourt RNAClean XP magnetic beads (Beckman Coulter, Cat. A63987) followed by second-strand synthesis reaction with NEBNext mRNA Second Strand Synthesis KIT (New England Biolabs, Cat. E6111S). Then linear amplification of cDNA was carried out through in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat. E2040S). IVT-amplified RNA was fragmented and purified again with Agencourt RNAClean XP magnetic beads. The second reverse transcription was done with PrimeScriptTM Reverse Transcriptase (Takara Clonetech, Cat. 2680A) followed with cDNA purification with Agencourt AMPure XP magnetic beads (Beckman Coulter, Cat.A63881). cDNA quantity was determined by qPCR on a fraction (5%) of purified cDNA. Final PCR amplification was done according to qPCR results and purified with Agencourt AMPure XP magnetic beads. Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen, Cat. Q32851). Library size was determined by Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat. 5067-4626). NextSeq 550 gds 303572763 GSM3572763 GEO Accession GSM3572763 SRX5267046 GSM3572764 SRP180361 SRS4270659 GSM3572764 RNA-Seq TRANSCRIPTOMIC cDNA Testicular single cells were encapsulated and barcoded with the inDrop microfluidics platform as instructed by the manufacturer (1CellBio). barcoded single-cell cDNA was purified with Agencourt RNAClean XP magnetic beads (Beckman Coulter, Cat. A63987) followed by second-strand synthesis reaction with NEBNext mRNA Second Strand Synthesis KIT (New England Biolabs, Cat. E6111S). Then linear amplification of cDNA was carried out through in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat. E2040S). IVT-amplified RNA was fragmented and purified again with Agencourt RNAClean XP magnetic beads. The second reverse transcription was done with PrimeScriptTM Reverse Transcriptase (Takara Clonetech, Cat. 2680A) followed with cDNA purification with Agencourt AMPure XP magnetic beads (Beckman Coulter, Cat.A63881). cDNA quantity was determined by qPCR on a fraction (5%) of purified cDNA. Final PCR amplification was done according to qPCR results and purified with Agencourt AMPure XP magnetic beads. Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen, Cat. Q32851). Library size was determined by Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat. 5067-4626). NextSeq 550 gds 303572764 GSM3572764 GEO Accession GSM3572764 SRX5267048 GSM3572765 SRP180361 SRS4270660 GSM3572765 RNA-Seq TRANSCRIPTOMIC cDNA Testicular single cells were encapsulated and barcoded with the inDrop microfluidics platform as instructed by the manufacturer (1CellBio). barcoded single-cell cDNA was purified with Agencourt RNAClean XP magnetic beads (Beckman Coulter, Cat. A63987) followed by second-strand synthesis reaction with NEBNext mRNA Second Strand Synthesis KIT (New England Biolabs, Cat. E6111S). Then linear amplification of cDNA was carried out through in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis kit (New England Biolabs, Cat. E2040S). IVT-amplified RNA was fragmented and purified again with Agencourt RNAClean XP magnetic beads. The second reverse transcription was done with PrimeScriptTM Reverse Transcriptase (Takara Clonetech, Cat. 2680A) followed with cDNA purification with Agencourt AMPure XP magnetic beads (Beckman Coulter, Cat.A63881). cDNA quantity was determined by qPCR on a fraction (5%) of purified cDNA. Final PCR amplification was done according to qPCR results and purified with Agencourt AMPure XP magnetic beads. Library concentration was determined by Qubit dsDNA HS Assay Kit (Invitrogen, Cat. Q32851). Library size was determined by Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat. 5067-4626). NextSeq 550 gds 303572765 GSM3572765 GEO Accession GSM3572765