SRX5273416 Vib-60-TDA-2 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273748 Vib-60-TDA-2 Vib-60-TDA-2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273417 Vib-60-DMSO-2 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273747 Vib-60-DMSO-2 Vib-60-DMSO-2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273418 Vib-30-TDA-2 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273749 Vib-30-TDA-2 Vib-30-TDA-2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273419 Vib-30-DMSO-2 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273750 Vib-30-DMSO-2 Vib-30-DMSO-2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273420 Vib-0-2 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273752 Vib-0-2 Vib-0-2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273421 Vib-60-TDA-1 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273751 Vib-60-TDA-1 Vib-60-TDA-1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273422 Vib-60-DMSO-1 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273754 Vib-60-DMSO-1 Vib-60-DMSO-1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273423 Vib-30-TDA-1 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273753 Vib-30-TDA-1 Vib-30-TDA-1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273424 Vib-30-DMSO-1 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273755 Vib-30-DMSO-1 Vib-30-DMSO-1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273425 Vib-0-1 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273756 Vib-0-1 Vib-0-1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273426 Vib-60-DMSO-3 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273758 Vib-60-DMSO-3 Vib-60-DMSO-3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273427 Vib-30-TDA-3 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273757 Vib-30-TDA-3 Vib-30-TDA-3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273428 Vib-30-DMSO-3 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273759 Vib-30-DMSO-3 Vib-30-DMSO-3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273429 Vib-0-3 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273760 Vib-0-3 Vib-0-3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000 SRX5273430 Vib-60-TDA-3 SRP180997 bp0 Vibrio vulnificus CMCP6 was grown in Mueller Hinton II Broth (MHB, Cation-Adjusted) at 37C. When OD600 reached 0.5 the cultures were split in two and equal amounts of fresh MHB was added. At OD600 = 0.5, equal volumes of TDA (final conc. 0.6 M) or DMSO (solvent of TDA). Samples for RNA sequencing were harvested from TDA-exposed and control cells (DMSO) at 0 min, 30 min and 60 min and mixed with RNAProtect Bacteria Reagent. Total RNA was extracted and depleted for rRNA leaving mRNA for library prep using the TruSeq RNA Sample preparation Kit v2. Lastly, each library was normalized to 10 nM and pooled yielding a final concentration of 10.3 nM with an average base pair size of 270. SRS4273761 Vib-60-TDA-3 Vib-60-TDA-3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2000