SRX5274720 WRSBa SRP181073 PRJNA512938 Nanopore sequencing was performed by using 1g of DNA template. End repair/dA tailing Ligation of Barcode Adapter Barcoding PCR End repair/dA tailing Blunt end Adapter Ligation Purification using AMPure XP bead binding Priming and loading the SpotON flow cell SRS4274924 SAMN10690897 WRSBa AMPLICON METAGENOMIC PCR MinION