SRX5389886 Zuchini SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377900 Zuchini Zuchini WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389887 Yellow_Zuchini SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377901 Yellow_Zuchini Yellow_Zuchini WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389888 Scallop SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377902 Scallop Scallop WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389889 Pumpkin SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377903 Pumpkin Pumpkin WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389890 Marrow SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377904 Marrow Marrow WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389891 Crookneck SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377905 Crookneck Crookneck WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389892 Cocozelle SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377906 Cocozelle Cocozelle WGS GENOMIC RANDOM PCR BGISEQ-500 SRX5389893 Acorn SRP186227 bp0 The extracted DNA was fragmented with a Bioruptor instrument (ThermoFisher Scientific, Waltham, MA, USA) to generate 200300 bp fragments. Libraries were then prepared as follows: first, the DNA fragments were subjected to end-repair and A-tailing; second, the resulting DNA was ligated with bubble-adapters that contained a barcode sequence, and then amplified with PCR.Qualified libraries were pooled together to form single-stranded DNA (ssDNA) circles and then DNA nanoballs were generated with rolling circle replication. The final DNA nanoballs were loaded onto a sequencing chip and were sequenced with a the BGISEQ-500 platform (BGI-Tianjin). SRS4377907 Acorn Acorn WGS GENOMIC RANDOM PCR BGISEQ-500