SRX5547699 GSM3680765 SRP188964 SRS4513304 GSM3680765 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680765 GSM3680765 GEO Accession GSM3680765 SRX5547700 GSM3680766 SRP188964 SRS4513305 GSM3680766 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680766 GSM3680766 GEO Accession GSM3680766 SRX5547701 GSM3680767 SRP188964 SRS4513306 GSM3680767 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680767 GSM3680767 GEO Accession GSM3680767 SRX5547702 GSM3680768 SRP188964 SRS4513307 GSM3680768 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680768 GSM3680768 GEO Accession GSM3680768 SRX5547703 GSM3680769 SRP188964 SRS4513308 GSM3680769 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680769 GSM3680769 GEO Accession GSM3680769 SRX5547704 GSM3680770 SRP188964 SRS4513309 GSM3680770 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680770 GSM3680770 GEO Accession GSM3680770 SRX5547705 GSM3680771 SRP188964 SRS4513310 GSM3680771 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680771 GSM3680771 GEO Accession GSM3680771 SRX5547706 GSM3680772 SRP188964 SRS4513311 GSM3680772 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from yeast cells using the hot phenol procedure. The amount of RNA was quantified in a Nanodrop 2000 Spectrophotometer (Thermo Scientific) and RNA integrity was checked by agarose gel electrophoresis. 5PSeq methods was performed as previously described (PMID: 26820793). 6µg of total RNA was used as input. In brief a RNA oligo (rP5_RND) containing an Illumina adaptor and unique molecular identifiers (UMI) was ligated to the intermediates of mRNA co-translation degradation (5'P). Ribosomal RNA was depleted using Ribo-Zero Magnetic Gold Kit (Illumina).Libraries were PCR amplified (14 cycles). Ampure beads size selected libraries with a length of 300-500 nt were sent for sequencing (llumina NextSeq 500 instrument). NextSeq 500 gds 303680772 GSM3680772 GEO Accession GSM3680772