SRX5547835 GSM3681103 SRP188974 SRS4513436 GSM3681103 RIP-Seq TRANSCRIPTOMIC other Crosslinked RIP was performed as previously described with minor modification(Gilbert and Svejstrup, 2006). Briefly, cells were first fixed with 0.3% formaldehyde for 10min followed by stopping crosslinking reaction with 0.2M glycine. Then the cells were lysed with FA lysis buffer (50 mM This.HCl, pH=7.5, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate) followed by sonication (10% AMP, 5s sonication, 5s pause, 10 cycles). The supernatant of the cell lysate was then mixed with antibody and protein A/G magnetic beads followed by incubation overnight at 4℃. Then the beads were washed two times of FA lysis buffer, FA500 buffer (50 mM This.HCl, pH=7.5, 500 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate), LiCl buffer (10 mM Tris HCl, pH 8, 250 mM LiCl, 0.5% (v/v) NP-40, 0.1% (w/v) sodium deoxycholate, 1 mM EDTA), and TE/100 mM NaCl buffer (10 mM Tris HCl, pH 8, 1 mM EDTA, 100 mM NaCl). The RNA was then eluted by elution buffer (100 mM Tris⋅Cl, pH 8, 10 mM EDTA, 1% (w/v) SDS) and extracted using Trizol (invitrogen). Paired-end sequencing libraries were prepared using the TruSeq Stranded Total Sample Preparation kit (Illumina) by the Mayo Clinic sequencing core facilities Illumina HiSeq 2000 gds 303681103 GSM3681103 GEO Accession GSM3681103 SRX5547836 GSM3681104 SRP188974 SRS4513437 GSM3681104 RIP-Seq TRANSCRIPTOMIC other Crosslinked RIP was performed as previously described with minor modification(Gilbert and Svejstrup, 2006). Briefly, cells were first fixed with 0.3% formaldehyde for 10min followed by stopping crosslinking reaction with 0.2M glycine. Then the cells were lysed with FA lysis buffer (50 mM This.HCl, pH=7.5, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate) followed by sonication (10% AMP, 5s sonication, 5s pause, 10 cycles). The supernatant of the cell lysate was then mixed with antibody and protein A/G magnetic beads followed by incubation overnight at 4℃. Then the beads were washed two times of FA lysis buffer, FA500 buffer (50 mM This.HCl, pH=7.5, 500 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate), LiCl buffer (10 mM Tris HCl, pH 8, 250 mM LiCl, 0.5% (v/v) NP-40, 0.1% (w/v) sodium deoxycholate, 1 mM EDTA), and TE/100 mM NaCl buffer (10 mM Tris HCl, pH 8, 1 mM EDTA, 100 mM NaCl). The RNA was then eluted by elution buffer (100 mM Tris⋅Cl, pH 8, 10 mM EDTA, 1% (w/v) SDS) and extracted using Trizol (invitrogen). Paired-end sequencing libraries were prepared using the TruSeq Stranded Total Sample Preparation kit (Illumina) by the Mayo Clinic sequencing core facilities Illumina HiSeq 2000 gds 303681104 GSM3681104 GEO Accession GSM3681104 SRX5547837 GSM3681105 SRP188974 SRS4513438 GSM3681105 RIP-Seq TRANSCRIPTOMIC other Crosslinked RIP was performed as previously described with minor modification(Gilbert and Svejstrup, 2006). Briefly, cells were first fixed with 0.3% formaldehyde for 10min followed by stopping crosslinking reaction with 0.2M glycine. Then the cells were lysed with FA lysis buffer (50 mM This.HCl, pH=7.5, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate) followed by sonication (10% AMP, 5s sonication, 5s pause, 10 cycles). The supernatant of the cell lysate was then mixed with antibody and protein A/G magnetic beads followed by incubation overnight at 4℃. Then the beads were washed two times of FA lysis buffer, FA500 buffer (50 mM This.HCl, pH=7.5, 500 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate), LiCl buffer (10 mM Tris HCl, pH 8, 250 mM LiCl, 0.5% (v/v) NP-40, 0.1% (w/v) sodium deoxycholate, 1 mM EDTA), and TE/100 mM NaCl buffer (10 mM Tris HCl, pH 8, 1 mM EDTA, 100 mM NaCl). The RNA was then eluted by elution buffer (100 mM Tris⋅Cl, pH 8, 10 mM EDTA, 1% (w/v) SDS) and extracted using Trizol (invitrogen). Paired-end sequencing libraries were prepared using the TruSeq Stranded Total Sample Preparation kit (Illumina) by the Mayo Clinic sequencing core facilities Illumina HiSeq 2000 gds 303681105 GSM3681105 GEO Accession GSM3681105 SRX5547838 GSM3681106 SRP188974 SRS4513439 GSM3681106 RIP-Seq TRANSCRIPTOMIC other Crosslinked RIP was performed as previously described with minor modification(Gilbert and Svejstrup, 2006). Briefly, cells were first fixed with 0.3% formaldehyde for 10min followed by stopping crosslinking reaction with 0.2M glycine. Then the cells were lysed with FA lysis buffer (50 mM This.HCl, pH=7.5, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate) followed by sonication (10% AMP, 5s sonication, 5s pause, 10 cycles). The supernatant of the cell lysate was then mixed with antibody and protein A/G magnetic beads followed by incubation overnight at 4℃. Then the beads were washed two times of FA lysis buffer, FA500 buffer (50 mM This.HCl, pH=7.5, 500 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate), LiCl buffer (10 mM Tris HCl, pH 8, 250 mM LiCl, 0.5% (v/v) NP-40, 0.1% (w/v) sodium deoxycholate, 1 mM EDTA), and TE/100 mM NaCl buffer (10 mM Tris HCl, pH 8, 1 mM EDTA, 100 mM NaCl). The RNA was then eluted by elution buffer (100 mM Tris⋅Cl, pH 8, 10 mM EDTA, 1% (w/v) SDS) and extracted using Trizol (invitrogen). Paired-end sequencing libraries were prepared using the TruSeq Stranded Total Sample Preparation kit (Illumina) by the Mayo Clinic sequencing core facilities Illumina HiSeq 2000 gds 303681106 GSM3681106 GEO Accession GSM3681106 SRX5547839 GSM3681107 SRP188974 SRS4513440 GSM3681107 RIP-Seq TRANSCRIPTOMIC other Crosslinked RIP was performed as previously described with minor modification(Gilbert and Svejstrup, 2006). Briefly, cells were first fixed with 0.3% formaldehyde for 10min followed by stopping crosslinking reaction with 0.2M glycine. Then the cells were lysed with FA lysis buffer (50 mM This.HCl, pH=7.5, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate) followed by sonication (10% AMP, 5s sonication, 5s pause, 10 cycles). The supernatant of the cell lysate was then mixed with antibody and protein A/G magnetic beads followed by incubation overnight at 4℃. Then the beads were washed two times of FA lysis buffer, FA500 buffer (50 mM This.HCl, pH=7.5, 500 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate), LiCl buffer (10 mM Tris HCl, pH 8, 250 mM LiCl, 0.5% (v/v) NP-40, 0.1% (w/v) sodium deoxycholate, 1 mM EDTA), and TE/100 mM NaCl buffer (10 mM Tris HCl, pH 8, 1 mM EDTA, 100 mM NaCl). The RNA was then eluted by elution buffer (100 mM Tris⋅Cl, pH 8, 10 mM EDTA, 1% (w/v) SDS) and extracted using Trizol (invitrogen). Paired-end sequencing libraries were prepared using the TruSeq Stranded Total Sample Preparation kit (Illumina) by the Mayo Clinic sequencing core facilities Illumina HiSeq 2000 gds 303681107 GSM3681107 GEO Accession GSM3681107 SRX5547840 GSM3681108 SRP188974 SRS4513441 GSM3681108 RIP-Seq TRANSCRIPTOMIC other Crosslinked RIP was performed as previously described with minor modification(Gilbert and Svejstrup, 2006). Briefly, cells were first fixed with 0.3% formaldehyde for 10min followed by stopping crosslinking reaction with 0.2M glycine. Then the cells were lysed with FA lysis buffer (50 mM This.HCl, pH=7.5, 140 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate) followed by sonication (10% AMP, 5s sonication, 5s pause, 10 cycles). The supernatant of the cell lysate was then mixed with antibody and protein A/G magnetic beads followed by incubation overnight at 4℃. Then the beads were washed two times of FA lysis buffer, FA500 buffer (50 mM This.HCl, pH=7.5, 500 mM NaCl, 1 mM EDTA, 1% (v/v) Triton X-100, 0.1% (w/v) sodium deoxycholate), LiCl buffer (10 mM Tris HCl, pH 8, 250 mM LiCl, 0.5% (v/v) NP-40, 0.1% (w/v) sodium deoxycholate, 1 mM EDTA), and TE/100 mM NaCl buffer (10 mM Tris HCl, pH 8, 1 mM EDTA, 100 mM NaCl). The RNA was then eluted by elution buffer (100 mM Tris⋅Cl, pH 8, 10 mM EDTA, 1% (w/v) SDS) and extracted using Trizol (invitrogen). Paired-end sequencing libraries were prepared using the TruSeq Stranded Total Sample Preparation kit (Illumina) by the Mayo Clinic sequencing core facilities Illumina HiSeq 2000 gds 303681108 GSM3681108 GEO Accession GSM3681108