SRX5552334 GSM3682096 SRP189085 SRS4517815 GSM3682096 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682096 GSM3682096 GEO Accession GSM3682096 SRX5552335 GSM3682097 SRP189085 SRS4517816 GSM3682097 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682097 GSM3682097 GEO Accession GSM3682097 SRX5552336 GSM3682098 SRP189085 SRS4517817 GSM3682098 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682098 GSM3682098 GEO Accession GSM3682098 SRX5552337 GSM3682099 SRP189085 SRS4517818 GSM3682099 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682099 GSM3682099 GEO Accession GSM3682099 SRX5552338 GSM3682100 SRP189085 SRS4517819 GSM3682100 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682100 GSM3682100 GEO Accession GSM3682100 SRX5552339 GSM3682101 SRP189085 SRS4517820 GSM3682101 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682101 GSM3682101 GEO Accession GSM3682101 SRX5552340 GSM3682102 SRP189085 SRS4517821 GSM3682102 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682102 GSM3682102 GEO Accession GSM3682102 SRX5552341 GSM3682103 SRP189085 SRS4517822 GSM3682103 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682103 GSM3682103 GEO Accession GSM3682103 SRX5552342 GSM3682104 SRP189085 SRS4517823 GSM3682104 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682104 GSM3682104 GEO Accession GSM3682104 SRX5552343 GSM3682105 SRP189085 SRS4517824 GSM3682105 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted using a standard hot acid phenol Total RNA was extracted from diploid yeast strains grown to mid-exponential phase (OD600 ~0.5) using a standard hot acid phenol protocol followed by chloroform extraction and ethanol precipitation. Afterwards, RNA was resuspended in DEPC-treated water and quantified on a NanoDrop spectrophotometer. Further quality control and ribosomal RNA evaluation was performed by analysis on an Agilent Bioanalyzer 2100 Nano RNA assay at the Protein and Nucleic Acid Facility (Stanford). For library generation, ERCC spike-in controls (Invitrogen) were added, and total RNA was reverse-transcribed by on-bead polyA-tail priming with Superscript IV (Invitrogen), and then fragmented on a Covaris E220 to a size range of 200–400 bp. Fragmented cDNA was end-repaired and A-tailed, and dual-index sequencing adaptors were ligated using the NEBNext Ultra II DNA Library Prep Kit. Prepared libraries were sequenced on the Illumina HiSeq 4000 platform at the Stanford Functional Genomics Facility using paired-end reads. Illumina HiSeq 4000 gds 303682105 GSM3682105 GEO Accession GSM3682105