SRX5552535 GSM3682113 SRP189089 SRS4517850 GSM3682113 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682113 GSM3682113 GEO Accession GSM3682113 SRX5552536 GSM3682114 SRP189089 SRS4517851 GSM3682114 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682114 GSM3682114 GEO Accession GSM3682114 SRX5552537 GSM3682115 SRP189089 SRS4517852 GSM3682115 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682115 GSM3682115 GEO Accession GSM3682115 SRX5552539 GSM3682116 SRP189089 SRS4517853 GSM3682116 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682116 GSM3682116 GEO Accession GSM3682116 SRX5552540 GSM3682117 SRP189089 SRS4517854 GSM3682117 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682117 GSM3682117 GEO Accession GSM3682117 SRX5552542 GSM3682118 SRP189089 SRS4517855 GSM3682118 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682118 GSM3682118 GEO Accession GSM3682118 SRX5552543 GSM3682119 SRP189089 SRS4517856 GSM3682119 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682119 GSM3682119 GEO Accession GSM3682119 SRX5552544 GSM3682120 SRP189089 SRS4517857 GSM3682120 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682120 GSM3682120 GEO Accession GSM3682120 SRX5552545 GSM3682121 SRP189089 SRS4517858 GSM3682121 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682121 GSM3682121 GEO Accession GSM3682121 SRX5552547 GSM3682122 SRP189089 SRS4517859 GSM3682122 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682122 GSM3682122 GEO Accession GSM3682122 SRX5552548 GSM3682123 SRP189089 SRS4517860 GSM3682123 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682123 GSM3682123 GEO Accession GSM3682123 SRX5552549 GSM3682124 SRP189089 SRS4517861 GSM3682124 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682124 GSM3682124 GEO Accession GSM3682124 SRX5552550 GSM3682125 SRP189089 SRS4517862 GSM3682125 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682125 GSM3682125 GEO Accession GSM3682125 SRX5552552 GSM3682126 SRP189089 SRS4517863 GSM3682126 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682126 GSM3682126 GEO Accession GSM3682126 SRX5552553 GSM3682127 SRP189089 SRS4517864 GSM3682127 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682127 GSM3682127 GEO Accession GSM3682127 SRX5552555 GSM3682128 SRP189089 SRS4517865 GSM3682128 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682128 GSM3682128 GEO Accession GSM3682128 SRX5552556 GSM3682129 SRP189089 SRS4517866 GSM3682129 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682129 GSM3682129 GEO Accession GSM3682129 SRX5552557 GSM3682130 SRP189089 SRS4517867 GSM3682130 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682130 GSM3682130 GEO Accession GSM3682130 SRX5552558 GSM3682131 SRP189089 SRS4517868 GSM3682131 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682131 GSM3682131 GEO Accession GSM3682131 SRX5552560 GSM3682132 SRP189089 SRS4517869 GSM3682132 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682132 GSM3682132 GEO Accession GSM3682132 SRX5552561 GSM3682133 SRP189089 SRS4517870 GSM3682133 ChIP-Seq GENOMIC ChIP RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682133 GSM3682133 GEO Accession GSM3682133 SRX5552563 GSM3682134 SRP189089 SRS4517871 GSM3682134 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682134 GSM3682134 GEO Accession GSM3682134 SRX5552564 GSM3682135 SRP189089 SRS4517872 GSM3682135 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682135 GSM3682135 GEO Accession GSM3682135 SRX5552566 GSM3682136 SRP189089 SRS4517873 GSM3682136 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682136 GSM3682136 GEO Accession GSM3682136 SRX5552567 GSM3682137 SRP189089 SRS4517874 GSM3682137 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682137 GSM3682137 GEO Accession GSM3682137 SRX5552569 GSM3682138 SRP189089 SRS4517875 GSM3682138 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682138 GSM3682138 GEO Accession GSM3682138 SRX5552570 GSM3682139 SRP189089 SRS4517876 GSM3682139 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682139 GSM3682139 GEO Accession GSM3682139 SRX5552572 GSM3682140 SRP189089 SRS4517877 GSM3682140 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682140 GSM3682140 GEO Accession GSM3682140 SRX5552573 GSM3682141 SRP189089 SRS4517878 GSM3682141 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682141 GSM3682141 GEO Accession GSM3682141 SRX5552575 GSM3682142 SRP189089 SRS4517879 GSM3682142 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682142 GSM3682142 GEO Accession GSM3682142 SRX5552577 GSM3682143 SRP189089 SRS4517880 GSM3682143 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682143 GSM3682143 GEO Accession GSM3682143 SRX5552579 GSM3682144 SRP189089 SRS4517881 GSM3682144 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682144 GSM3682144 GEO Accession GSM3682144 SRX5552581 GSM3682145 SRP189089 SRS4517882 GSM3682145 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682145 GSM3682145 GEO Accession GSM3682145 SRX5552583 GSM3682146 SRP189089 SRS4517883 GSM3682146 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682146 GSM3682146 GEO Accession GSM3682146 SRX5552585 GSM3682147 SRP189089 SRS4517884 GSM3682147 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682147 GSM3682147 GEO Accession GSM3682147 SRX5552587 GSM3682148 SRP189089 SRS4517885 GSM3682148 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682148 GSM3682148 GEO Accession GSM3682148 SRX5552589 GSM3682149 SRP189089 SRS4517886 GSM3682149 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682149 GSM3682149 GEO Accession GSM3682149 SRX5552591 GSM3682150 SRP189089 SRS4517887 GSM3682150 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682150 GSM3682150 GEO Accession GSM3682150 SRX5552593 GSM3682151 SRP189089 SRS4517888 GSM3682151 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682151 GSM3682151 GEO Accession GSM3682151 SRX5552595 GSM3682152 SRP189089 SRS4517889 GSM3682152 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682152 GSM3682152 GEO Accession GSM3682152 SRX5552597 GSM3682153 SRP189089 SRS4517890 GSM3682153 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682153 GSM3682153 GEO Accession GSM3682153 SRX5552599 GSM3682154 SRP189089 SRS4517891 GSM3682154 RNA-Seq TRANSCRIPTOMIC cDNA RNA-Seq: Total RNA from 3 replicates (i.e. livers from 3 mice at each CT and from NS mice; see above) was extracted from liver pieces using the RNeasy Mini Kit (Qiagen). DNase treatment was performed with RQ1 RNase-Free DNase (M101, Promega). ChiP-Seq: ChIP of LMNB1 was done as described {Rønningen et al 2015 Genome Res 25: 1825-1835}. Snap-frozen liver tissue pieces (40-50 mg) were thawed on ice and minced on ice for 30 sec. Minced tissue was resuspended in PBS with 1 mM PMSF and protease inhibitors, and homogenized by 7-10 strokes in a 2-ml Dounce homogenizer using pestle 'B'. Samples were centrifuged at 400 g and supernatants discarded. Pellets were resuspended in PBS containing 1% formaldehyde and cross-linking allowed to occur for 10 min at room temperature. Cross-linked samples were sedimented and lysed in RIPA buffer (140 mM NaCl, 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 1% Triton X-100, 1% SDS, 0.1% sodium deoxycholate, 1 mM PMSF, protease inhibitors). To generate 200-500 bp DNA fragments, cells were sonicated 4 times 10 min in a Bioruptor (Diagenode). After sedimentation, the supernatant was diluted 10-fold in RIPA and incubated with anti-LMNB1 antibodies (10 µg; Abcam ab16048) coupled to Dynabeads Protein G (Invitrogen) overnight at 4oC. ChIP samples were washed 3 times in ice-cold RIPA. Cross-links were reversed and DNA eluted for 6 h at 68°C in elution buffer (50 mM NaCl, 20 mM Tris-HCl, pH 7.5, 5 mM EDTA, 1% SDS) containing 40 ng/ml proteinase K. DNA was extracted and libraries prepared and sequenced on an Illumina HiSeq2500. ChIP-seq and RNA-seq sequencing libraries were prepared according to Illumina protocols for sequencing on a HSeq2500 at the Norwegian Sequencing Center (Oslo University Hospital). Illumina HiSeq 2500 gds 303682154 GSM3682154 GEO Accession GSM3682154