SRX5576255 Om05root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538280 SAMN11251680 Om05root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576256 Oo08root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538281 SAMN11251687 Oo08root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576257 Oo10root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538282 SAMN11251688 Oo10root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576258 So05root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538283 SAMN11251696 So05root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576259 So04root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538284 SAMN11251695 So04root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576260 So03root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538285 SAMN11251694 So03root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576261 Sm09root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538286 SAMN11251693 Sm09root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576262 Om08rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538287 SAMN11251652 Om08rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576263 Om06rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538288 SAMN11251651 Om06rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576264 Om05rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538289 SAMN11251650 Om05rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576265 Om03rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538290 SAMN11251649 Om03rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576266 Oo06rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538291 SAMN11251656 Oo06rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576267 Oo05rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538292 SAMN11251655 Oo05rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576268 Oo04rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538293 SAMN11251654 Oo04rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576269 Om10rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538294 SAMN11251653 Om10rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576270 Oo10rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538295 SAMN11251658 Oo10rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576271 Oo08rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538296 SAMN11251657 Oo08rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576272 So07rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538297 SAMN11251667 So07rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576273 So09rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538298 SAMN11251668 So09rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576274 Sm09rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538299 SAMN11251663 Sm09rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576275 So03rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538300 SAMN11251664 So03rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576276 So04rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538301 SAMN11251665 So04rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576277 So05rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538302 SAMN11251666 So05rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576278 Sm02rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538303 SAMN11251659 Sm02rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576279 Sm04rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538304 SAMN11251660 Sm04rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576280 Sm05rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538305 SAMN11251661 Sm05rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576281 Sm08rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538306 SAMN11251662 Sm08rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576282 So09bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538307 SAMN11251638 So09bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576283 So07bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538308 SAMN11251637 So07bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576284 Sm04bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538309 SAMN11251630 Sm04bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576285 Sm02bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538310 SAMN11251629 Sm02bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576286 Sm08bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538311 SAMN11251632 Sm08bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576287 Sm05bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538312 SAMN11251631 Sm05bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576288 So03bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538313 SAMN11251634 So03bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576289 Sm09bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538314 SAMN11251633 Sm09bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576290 So05bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538315 SAMN11251636 So05bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576291 So04bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538316 SAMN11251635 So04bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576292 Eo09rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538317 SAMN11251647 Eo09rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576293 Eo10rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538318 SAMN11251648 Eo10rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576294 Em03rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538319 SAMN11251641 Em03rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576295 Em05rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538320 SAMN11251642 Em05rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576296 Em01rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538321 SAMN11251639 Em01rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576297 Em02rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538322 SAMN11251640 Em02rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576298 Eo05rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538323 SAMN11251645 Eo05rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576299 Eo07rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538324 SAMN11251646 Eo07rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576300 Em08rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538325 SAMN11251643 Em08rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576301 Eo03rhizo SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538326 SAMN11251644 Eo03rhizo AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576302 So09root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538327 SAMN11251698 So09root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576303 So07root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538328 SAMN11251697 So07root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576304 OoStart SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538329 SAMN11251710 OoStart AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576305 OmStart SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538330 SAMN11251709 OmStart AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576306 SoStart SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538331 SAMN11251712 SoStart AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576307 SmStart SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538332 SAMN11251711 SmStart AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576308 Sm08root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538333 SAMN11251692 Sm08root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576309 Sm05root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538334 SAMN11251691 Sm05root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576310 Sm04root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538335 SAMN11251690 Sm04root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576311 Sm02root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538336 SAMN11251689 Sm02root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576312 Em03bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538337 SAMN11251611 Em03bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576313 Em05bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538338 SAMN11251612 Em05bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576314 Em01bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538339 SAMN11251609 Em01bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576315 Em02bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538340 SAMN11251610 Em02bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576316 Eo05bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538341 SAMN11251615 Eo05bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576317 Eo07bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538342 SAMN11251616 Eo07bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576318 Em08bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538343 SAMN11251613 Em08bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576319 Eo03bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538344 SAMN11251614 Eo03bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576320 Eo09bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538345 SAMN11251617 Eo09bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576321 Eo10bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538346 SAMN11251618 Eo10bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576322 Om03bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538347 SAMN11251619 Om03bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576323 Om05bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538348 SAMN11251620 Om05bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576324 Om06bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538349 SAMN11251621 Om06bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576325 Om08bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538350 SAMN11251622 Om08bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576326 Om10bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538351 SAMN11251623 Om10bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576327 Oo04bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538352 SAMN11251624 Oo04bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576328 Oo05bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538353 SAMN11251625 Oo05bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576329 Oo06bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538354 SAMN11251626 Oo06bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576330 Oo08bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538355 SAMN11251627 Oo08bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576331 Oo10bulk SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538356 SAMN11251628 Oo10bulk AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576332 EmStart SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538357 SAMN11251707 EmStart AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576333 EoStart SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538358 SAMN11251708 EoStart AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576334 NegExt01 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538359 SAMN11251699 NegExt01 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576335 NegExt02 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538360 SAMN11251700 NegExt02 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576336 NegExt03 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538361 SAMN11251701 NegExt03 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576337 NegExt04 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538362 SAMN11251702 NegExt04 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576338 NegPCR1 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538363 SAMN11251703 NegPCR1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576339 NegPCR2 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538364 SAMN11251704 NegPCR2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576340 NegPCR3 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538365 SAMN11251705 NegPCR3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576341 NegPCR4 SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538366 SAMN11251706 NegPCR4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576342 Eo03root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538367 SAMN11251674 Eo03root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576343 Em08root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538368 SAMN11251673 Em08root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576344 Eo07root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538369 SAMN11251676 Eo07root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576345 Eo05root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538371 SAMN11251675 Eo05root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576346 Em02root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538370 SAMN11251670 Em02root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576347 Em01root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538372 SAMN11251669 Em01root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576348 Em05root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538373 SAMN11251672 Em05root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576349 Em03root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538374 SAMN11251671 Em03root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576350 Eo10root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538375 SAMN11251678 Eo10root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576351 Eo09root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538376 SAMN11251677 Eo09root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576352 Oo05root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538377 SAMN11251685 Oo05root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576353 Oo06root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538378 SAMN11251686 Oo06root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576354 Om10root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538379 SAMN11251683 Om10root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576355 Oo04root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538380 SAMN11251684 Oo04root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576356 Om06root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538381 SAMN11251681 Om06root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576357 Om08root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538382 SAMN11251682 Om08root AMPLICON METAGENOMIC PCR Illumina MiSeq SRX5576358 Om03root SRP189444 PRJNA528968 The BioProject collection stems from a mesocosm experiment on the influence of bacterial diversity on the N-mineralisation capabilities of the soil microbial diversity. DNA was extracted using the NuceloSpin Soil DNA extraction kit (Macherey-Nagel GmbH & Co. KG, Dren, Germany) following the manufacturers instructions. Per sample, 0.5 g of fresh soil were homogenized with the provided SL2 lysis buffer in a Precellys 24 device (Bertin Instruments, Montigny-le-Bretonneux, France). Negative extraction controls were done using empty tubes adding only the kit reagents. The bacterial community of bulk soil and rhizosphere soil as well as the plant-associated bacterial community were characterised using Illumina MiSeq Sequencing of PCR amplicons of the bacterial 16S rRNA gene (V3-V4 region) using the primers 335F (5-CADACTCCTACGGGAGGC-3) and 769R (5-ATCCTGTTTGMTMCCCVCRC-3). Ten ng of input DNA were used, for negative control only sterilised water was added. The PCR program included an initial denaturation step of 30 s at 98 C followed by 25 cycles denaturation 10 s 98 C; annealing 30 s 60 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. Ten nanogrammes of amplicon DNA per sample were indexed for multiplexed short-read sequencing by use of the Nextera XT Index Kit v2 (Illumina Inc., San Diego, US-CA) following the manufacturers instructions. The PCR program consisted of an initial denaturation step of 30 s at 98 C followed by 8 cycles denaturation 10 s 98 C; annealing 30 s 55 C; elongation 30 s 72 C and a final elongation time of 5 min at 72 C. After quality checks as described above, the indexed amplicon DNA was diluted to 4 nM and pooled equimolarly for sequencing with a MiSeq System (Illumina) using the MiSeq Reagent Kit v3 (600 cycle) for paired end sequencing following the manufacturers instructions. A final amount of 8 pmol DNA was loaded. As a positive control, PhiX (Illumina) was used as a spike-in. The sequence data were de-multiplexed on the Illumina platform. Thereafter, adapter sequences were removed using the software cutadapt version 1.16 (Martin 2011). SRS4538383 SAMN11251679 Om03root AMPLICON METAGENOMIC PCR Illumina MiSeq