SRX5580245 GSM3688219 SRP189525 SRS4541939 GSM3688219 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688219 GSM3688219 GEO Accession GSM3688219 SRX5580246 GSM3688220 SRP189525 SRS4541938 GSM3688220 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688220 GSM3688220 GEO Accession GSM3688220 SRX5580247 GSM3688221 SRP189525 SRS4541940 GSM3688221 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688221 GSM3688221 GEO Accession GSM3688221 SRX5580248 GSM3688222 SRP189525 SRS4541942 GSM3688222 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688222 GSM3688222 GEO Accession GSM3688222 SRX5580249 GSM3688223 SRP189525 SRS4541941 GSM3688223 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688223 GSM3688223 GEO Accession GSM3688223 SRX5580250 GSM3688224 SRP189525 SRS4541943 GSM3688224 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688224 GSM3688224 GEO Accession GSM3688224 SRX5580251 GSM3688225 SRP189525 SRS4541945 GSM3688225 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688225 GSM3688225 GEO Accession GSM3688225 SRX5580252 GSM3688226 SRP189525 SRS4541944 GSM3688226 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688226 GSM3688226 GEO Accession GSM3688226 SRX5580253 GSM3688227 SRP189525 SRS4541946 GSM3688227 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688227 GSM3688227 GEO Accession GSM3688227 SRX5580254 GSM3688228 SRP189525 SRS4541947 GSM3688228 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688228 GSM3688228 GEO Accession GSM3688228 SRX5580255 GSM3688229 SRP189525 SRS4541948 GSM3688229 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688229 GSM3688229 GEO Accession GSM3688229 SRX5580256 GSM3688230 SRP189525 SRS4541951 GSM3688230 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688230 GSM3688230 GEO Accession GSM3688230 SRX5580257 GSM3688231 SRP189525 SRS4541949 GSM3688231 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688231 GSM3688231 GEO Accession GSM3688231 SRX5580258 GSM3688232 SRP189525 SRS4541952 GSM3688232 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688232 GSM3688232 GEO Accession GSM3688232 SRX5580259 GSM3688233 SRP189525 SRS4541950 GSM3688233 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688233 GSM3688233 GEO Accession GSM3688233 SRX5580260 GSM3688234 SRP189525 SRS4541953 GSM3688234 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688234 GSM3688234 GEO Accession GSM3688234 SRX5580261 GSM3688235 SRP189525 SRS4541954 GSM3688235 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688235 GSM3688235 GEO Accession GSM3688235 SRX5580262 GSM3688236 SRP189525 SRS4541955 GSM3688236 RNA-Seq TRANSCRIPTOMIC cDNA Bone marrow neutrophils were FACS sorted and subdivided into three cohorts: unstimulated (immediately post-sort), or cultured in GM-CSF-containing media for 4h or 24h. Cells were lysed in RLT buffer, RNA extracted using Rneasy Plus Mini Kit (QIAGEN). Next-generation sequencing libraries were created with 100 ng of RNA from samples with distinct 18S and 28S peaks and RNA Integrity Number (RIN) values greater 7, using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina New England Biolabs) according to the manufacturer's protocol. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 303688236 GSM3688236 GEO Accession GSM3688236