<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY center_name="BioProject" alias="PRJNA529454" accession="SRP189859">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP189859</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject" label="primary">PRJNA529454</EXTERNAL_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>Assembly Free Viral Genomes from Station ALOHA</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Metagenomics"/>
      <STUDY_ABSTRACT>We developed an assembly-free, single-molecule nanopore sequencing approach enabling direct recovery of high-quality viral genome sequences from environmental samples. Applied to samples from Station ALOHA in the North Pacific Subtropical Gyre, the method yielded full-length double-stranded DNA virus genome sequences and revealed viruses not fully recovered using short read assembly approaches in the same samples. Additionally, novel virus-like DNA sequences were discovered, whose structures, gene contents and concatemer lengths suggested they represent phage-inducible chromosomal islands packaged in discrete concatemeric lengths within phage particles.</STUDY_ABSTRACT>
      <CENTER_PROJECT_NAME>marine plankton metagenome</CENTER_PROJECT_NAME>
    </DESCRIPTOR>
    <STUDY_LINKS>
      <STUDY_LINK>
        <XREF_LINK>
          <DB>pubmed</DB>
          <ID>32075851</ID>
        </XREF_LINK>
      </STUDY_LINK>
      <STUDY_LINK>
        <URL_LINK>
          <LABEL>SCOPE Falkor Cruise</LABEL>
          <URL>http://scope.soest.hawaii.edu/data/falkor2018/falkor2018.html</URL>
        </URL_LINK>
      </STUDY_LINK>
      <STUDY_LINK>
        <URL_LINK>
          <LABEL>SCOPE HOE Legacy II Cruise</LABEL>
          <URL>http://scope.soest.hawaii.edu/data/hoelegacy/</URL>
        </URL_LINK>
      </STUDY_LINK>
    </STUDY_LINKS>
  </STUDY>
</STUDY_SET>
