SRX5619090 WTCHG_337912_239177 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_337912_239177 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619091 WTCHG_337911_239177 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_337911_239177 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619092 WTCHG_337910_239177 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_337910_239177 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619093 WTCHG_336861_239177 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_336861_239177 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619094 WTCHG_337912_236141 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_337912_236141 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619095 WTCHG_337911_236141 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_337911_236141 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619096 WTCHG_337910_236141 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_337910_236141 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619097 WTCHG_336861_236141 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562509 Effluent WTCHG_336861_236141 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619098 WTCHG_337910_245154 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_337910_245154 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619099 WTCHG_336861_245154 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_336861_245154 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619100 WTCHG_337910_246166 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_337910_246166 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619101 WTCHG_336861_246166 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_336861_246166 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619102 WTCHG_337912_238165 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_337912_238165 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619103 WTCHG_337911_238165 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_337911_238165 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619104 WTCHG_337910_238165 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_337910_238165 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619105 WTCHG_336861_238165 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_336861_238165 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619106 WTCHG_337912_245154 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_337912_245154 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619107 WTCHG_337911_245154 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562510 Pig caeca WTCHG_337911_245154 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619108 WTCHG_337912_246166 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_337912_246166 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619109 WTCHG_337911_246166 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_337911_246166 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619110 WTCHG_337911_237153 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_337911_237153 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619111 WTCHG_337912_237153 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_337912_237153 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619112 WTCHG_336861_237153 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_336861_237153 WGS METAGENOMIC RANDOM Illumina HiSeq 4000 SRX5619113 WTCHG_337910_237153 SRP189676 PRJNA529503 Metagenomic extractions on all samples were performed using the MoBio PowerSoil DNA Isolation Kit (Qiagen, Venlo, Netherlands), as per the manufacturers protocol, and including a beadbeating step of two 40 second cycles at 6 m/s in lysing matrix E. 12.5 ng of naked Thermus thermophilus DNA (reference strain HB27, Collection number ATCC BAA-163, ordered from DSMZ, Germany) was added to each sample in the PowerBead tube at the start of the experiment, prior to the addition of Solution C1 of the DNA Isolation Kit. The rationale for this was to enable subsequent normalisation to the number of T. thermophilus genomes sequenced to adjust for varying amounts of sample input, and extraction bias. SRS4562511 Upstream sediment WTCHG_337910_237153 WGS METAGENOMIC RANDOM Illumina HiSeq 4000