SRX5620735 SG7 SRP190021 PRJNA530185 We use Picogreen (Invitrogen) to quantitate genomic DNA samples. DNA is sheared using a Covaris and libraries are constructed with the Nugen Ovation Ultralow Library protocol. We aim for an insert size of 400 bp to maximize data. Amplified libraries are visualized on an Agilent DNA1000 chip or Caliper HiSens Bioanalyzer assay, pooled at equimolar concentrations based on these results, and size selected using a Sage PippinPrep 2% cassette. The library pool is quantified using a Kapa Biosystems qPCR library quantification protocol, then sequenced on the Illumina NextSeq in a 2x150 paired-end sequencing run using dedicated read indexing. The samples are demultiplexed with bcl2fastq. SRS4564030 SAMN11303416 SG7 WGS METAGENOMIC RANDOM NextSeq 500 SRX5620736 WM1 SRP190021 PRJNA530185 We use Picogreen (Invitrogen) to quantitate genomic DNA samples. DNA is sheared using a Covaris and libraries are constructed with the Nugen Ovation Ultralow Library protocol. We aim for an insert size of 400 bp to maximize data. Amplified libraries are visualized on an Agilent DNA1000 chip or Caliper HiSens Bioanalyzer assay, pooled at equimolar concentrations based on these results, and size selected using a Sage PippinPrep 2% cassette. The library pool is quantified using a Kapa Biosystems qPCR library quantification protocol, then sequenced on the Illumina NextSeq in a 2x150 paired-end sequencing run using dedicated read indexing. The samples are demultiplexed with bcl2fastq. SRS4564031 SAMN11303415 WM1 WGS METAGENOMIC RANDOM NextSeq 500 SRX5620737 WM7 SRP190021 PRJNA530185 We use Picogreen (Invitrogen) to quantitate genomic DNA samples. DNA is sheared using a Covaris and libraries are constructed with the Nugen Ovation Ultralow Library protocol. We aim for an insert size of 400 bp to maximize data. Amplified libraries are visualized on an Agilent DNA1000 chip or Caliper HiSens Bioanalyzer assay, pooled at equimolar concentrations based on these results, and size selected using a Sage PippinPrep 2% cassette. The library pool is quantified using a Kapa Biosystems qPCR library quantification protocol, then sequenced on the Illumina NextSeq in a 2x150 paired-end sequencing run using dedicated read indexing. The samples are demultiplexed with bcl2fastq. SRS4564032 SAMN11303414 WM7 WGS METAGENOMIC RANDOM NextSeq 500