SRX5620750 GSM3701188 SRP190036 SRS4566893 GSM3701188 ChIP-Seq GENOMIC ChIP Compound- or vehicle-treated cells were crosslinked with 1% formaldehyde for 8 minutes and quenched with glycine for 5 minutes, lysed in 2X lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1% Triton X-100, 0.1% Na Deoxycholate, 5mM CaCl2 and protease inhibitors) for 20 minutes on ice, and digested with 100u MNase (New England Biolabs M0247) for 10 minutes at 37°C. The MNase digestion was terminated by addition of 10mM EDTA. Chromatin was incubated with the anti-H3K4me3 antibody (Millipore 07-473, RRID: AB_1977252) overnight at 4°C, followed by incubation with Protein G beads (Invitrogen 10004D) for 2 hours at 4°C. The beads were washed with PBS (6x) and samples were eluted in EB (10mM Tris-HCl pH8, 5mM EDTA, 300mM NaCl, 0.1% SDS) supplemented with Proteinase K (New England Biolabs P8107S). SPRI beads were used for clean-up and yield was measured using Qubit Fluorimeter. Libraries were prepared using NEBNExt® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs E7645S). Libraries were sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads. Illumina HiSeq 2000 gds 303701188 GSM3701188 GEO Accession GSM3701188 SRX5620751 GSM3701189 SRP190036 SRS4566894 GSM3701189 ChIP-Seq GENOMIC ChIP Compound- or vehicle-treated cells were crosslinked with 1% formaldehyde for 8 minutes and quenched with glycine for 5 minutes, lysed in 2X lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1% Triton X-100, 0.1% Na Deoxycholate, 5mM CaCl2 and protease inhibitors) for 20 minutes on ice, and digested with 100u MNase (New England Biolabs M0247) for 10 minutes at 37°C. The MNase digestion was terminated by addition of 10mM EDTA. Chromatin was incubated with the anti-H3K4me3 antibody (Millipore 07-473, RRID: AB_1977252) overnight at 4°C, followed by incubation with Protein G beads (Invitrogen 10004D) for 2 hours at 4°C. The beads were washed with PBS (6x) and samples were eluted in EB (10mM Tris-HCl pH8, 5mM EDTA, 300mM NaCl, 0.1% SDS) supplemented with Proteinase K (New England Biolabs P8107S). SPRI beads were used for clean-up and yield was measured using Qubit Fluorimeter. Libraries were prepared using NEBNExt® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs E7645S). Libraries were sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads. Illumina HiSeq 2000 gds 303701189 GSM3701189 GEO Accession GSM3701189 SRX5620752 GSM3701190 SRP190036 SRS4566895 GSM3701190 ChIP-Seq GENOMIC ChIP Compound- or vehicle-treated cells were crosslinked with 1% formaldehyde for 8 minutes and quenched with glycine for 5 minutes, lysed in 2X lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1% Triton X-100, 0.1% Na Deoxycholate, 5mM CaCl2 and protease inhibitors) for 20 minutes on ice, and digested with 100u MNase (New England Biolabs M0247) for 10 minutes at 37°C. The MNase digestion was terminated by addition of 10mM EDTA. Chromatin was incubated with the anti-H3K4me3 antibody (Millipore 07-473, RRID: AB_1977252) overnight at 4°C, followed by incubation with Protein G beads (Invitrogen 10004D) for 2 hours at 4°C. The beads were washed with PBS (6x) and samples were eluted in EB (10mM Tris-HCl pH8, 5mM EDTA, 300mM NaCl, 0.1% SDS) supplemented with Proteinase K (New England Biolabs P8107S). SPRI beads were used for clean-up and yield was measured using Qubit Fluorimeter. Libraries were prepared using NEBNExt® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs E7645S). Libraries were sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads. Illumina HiSeq 2000 gds 303701190 GSM3701190 GEO Accession GSM3701190 SRX5620753 GSM3701191 SRP190036 SRS4566897 GSM3701191 ChIP-Seq GENOMIC ChIP Compound- or vehicle-treated cells were crosslinked with 1% formaldehyde for 8 minutes and quenched with glycine for 5 minutes, lysed in 2X lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1% Triton X-100, 0.1% Na Deoxycholate, 5mM CaCl2 and protease inhibitors) for 20 minutes on ice, and digested with 100u MNase (New England Biolabs M0247) for 10 minutes at 37°C. The MNase digestion was terminated by addition of 10mM EDTA. Chromatin was incubated with the anti-H3K4me3 antibody (Millipore 07-473, RRID: AB_1977252) overnight at 4°C, followed by incubation with Protein G beads (Invitrogen 10004D) for 2 hours at 4°C. The beads were washed with PBS (6x) and samples were eluted in EB (10mM Tris-HCl pH8, 5mM EDTA, 300mM NaCl, 0.1% SDS) supplemented with Proteinase K (New England Biolabs P8107S). SPRI beads were used for clean-up and yield was measured using Qubit Fluorimeter. Libraries were prepared using NEBNExt® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs E7645S). Libraries were sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads. Illumina HiSeq 2000 gds 303701191 GSM3701191 GEO Accession GSM3701191 SRX5620754 GSM3701192 SRP190036 SRS4566896 GSM3701192 ChIP-Seq GENOMIC ChIP Compound- or vehicle-treated cells were crosslinked with 1% formaldehyde for 8 minutes and quenched with glycine for 5 minutes, lysed in 2X lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1% Triton X-100, 0.1% Na Deoxycholate, 5mM CaCl2 and protease inhibitors) for 20 minutes on ice, and digested with 100u MNase (New England Biolabs M0247) for 10 minutes at 37°C. The MNase digestion was terminated by addition of 10mM EDTA. Chromatin was incubated with the anti-H3K4me3 antibody (Millipore 07-473, RRID: AB_1977252) overnight at 4°C, followed by incubation with Protein G beads (Invitrogen 10004D) for 2 hours at 4°C. The beads were washed with PBS (6x) and samples were eluted in EB (10mM Tris-HCl pH8, 5mM EDTA, 300mM NaCl, 0.1% SDS) supplemented with Proteinase K (New England Biolabs P8107S). SPRI beads were used for clean-up and yield was measured using Qubit Fluorimeter. Libraries were prepared using NEBNExt® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs E7645S). Libraries were sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads. Illumina HiSeq 2000 gds 303701192 GSM3701192 GEO Accession GSM3701192 SRX5620755 GSM3701193 SRP190036 SRS4566899 GSM3701193 ChIP-Seq GENOMIC ChIP Compound- or vehicle-treated cells were crosslinked with 1% formaldehyde for 8 minutes and quenched with glycine for 5 minutes, lysed in 2X lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1% Triton X-100, 0.1% Na Deoxycholate, 5mM CaCl2 and protease inhibitors) for 20 minutes on ice, and digested with 100u MNase (New England Biolabs M0247) for 10 minutes at 37°C. The MNase digestion was terminated by addition of 10mM EDTA. Chromatin was incubated with the anti-H3K4me3 antibody (Millipore 07-473, RRID: AB_1977252) overnight at 4°C, followed by incubation with Protein G beads (Invitrogen 10004D) for 2 hours at 4°C. The beads were washed with PBS (6x) and samples were eluted in EB (10mM Tris-HCl pH8, 5mM EDTA, 300mM NaCl, 0.1% SDS) supplemented with Proteinase K (New England Biolabs P8107S). SPRI beads were used for clean-up and yield was measured using Qubit Fluorimeter. Libraries were prepared using NEBNExt® Ultra™ II DNA Library Prep Kit for Illumina (New England Biolabs E7645S). Libraries were sequenced on an Illumina Hi-Seq 2000 for single-end 50bp reads. Illumina HiSeq 2000 gds 303701193 GSM3701193 GEO Accession GSM3701193