SRX5625374 RCr_3 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570709 RCr_3 RCr_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625375 RCr_2 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570711 RCr_2 RCr_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625376 TC_2 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570712 TC_2 TC_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625377 TC_1 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570710 TC_1 TC_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625378 TCr_3 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570713 TCr_3 TCr_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625379 RC_1 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570714 RC_1 RC_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625380 RCr_1 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570716 RCr_1 RCr_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625381 RC_3 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570717 RC_3 RC_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625382 RC_2 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570715 RC_2 RC_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625383 TCr_1 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570718 TCr_1 TCr_1 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625384 TC_3 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570719 TC_3 TC_3 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500 SRX5625385 TCr_2 SRP190178 bp0 A total amount of 1.5 mg total RNA per mycelium sample was used as input for RNA sample preparation and three repetitions per treatment. Sequencing libraries were generated using an NEBNext Ultra RNA library prep kit for Illumina (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. Briefly, mRNA was purified from total RNA using polyT oligo-attached magnetic beads. Fragmentation was conducted using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer (5). First-strand cDNA was synthesized using random hexamer primers and M-MuLV reverse transcriptase (RNase H-). Second-strand cDNA synthesis was subsequently performed using DNA polymerase I. The remaining overhangs were converted into blunt ends using exonuclease/polymerase activities. After adenylation of the 3 ends of the DNA fragments, NEBNext adaptors with hairpin loop structures were ligated to prepare for hybridization. To select cDNA fragments of preferentially 150-200 bp in length, the library fragments were purified with an AMPure XP system (Beckman Coulter, Beverly, USA). Then, 3 L of USER enzyme (NEB, USA) was used with size-selected, adaptor-ligated cDNA at 37 for 15 min followed by 5 min at 95 before PCR. Then, PCR was performed with Phusion High-Fidelity DNA polymerase, universal PCR primers, and an Index (X) primer. Finally, PCR products were purified (AMPure XP system), and library quality was assessed on the Agilent Bioanalyzer 2100 system. After the library quality inspection qualified, we performed the paired-end sequencing on an Illumina Hiseq 2500 at the (lc-bio, China) following the vendor's recommended protocol. SRS4570720 TCr_2 TCr_2 RNA-Seq TRANSCRIPTOMIC Oligo-dT Illumina HiSeq 2500