SRX5649549 Nup93_ChIP_Replicate-2 SRP191222 bp0 Crosslinking 1% paraformaldehyde for 10 min, nuclear purification with 0.5% NP40, DNA solubilization and sonication in 1% SDS using Covaris sonicator, followed by Immunoprecipitation with following antibodies 1.Anti-Nup93 antibody (sc-292099)- 2g/ 100g of chromatin, 2. Normal Rabbit IgG (Millipore 12-370). Arpoximately 20-30 ng of DNA was used for library preparation. ChIP-Seq libraries for sequencing were constructed according to the NEXTflex ChIP-Seq library protocol outlined in NEXTflex ChIP-Seq Kit - 5143-01. Briefly, DNA was subjected to a series of enzymatic reactions that repair frayed ends, phosphorylate the fragments, add a single nucleotide A overhang and ligate adaptors (NEXTflex ChIP Barcodes-48 kit). The libraries are enriched using PCR (5 cycles), fragments were size selected using a 2 % Low melting agarose gel and purified using MinElute Gel Extraction Kit (QIAGEN). The libraries were further enriched using PCR (14 cycles), post PCR cleanup was performed using Agencourt AMPURE XP beads (Beckman Coulter #A63881). The prepared libraries were quantified using Qubit fluorometer and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS4594914 Nup93_ChIP seq Nup93_ChIP_Replicate-2 ChIP-Seq GENOMIC ChIP Illumina HiSeq 2500 SRX5649550 Nup93_Input SRP191222 bp0 Cross-linking of cells using 1% paraformaldehyde for 10 min, nuclear purification with 0.5% NP40, DNA solubilization and sonication in 1% SDS using Covaris sonicator, followed by Immunoprecipitation with following antibodies 1.Anti-Nup93 antibody (sc-292099)- 2g/ 100g of chromatin, 2. Normal Rabbit IgG (Millipore 12-370). Arpoximately 20-30 ng of DNA was used for library preparation. ChIP-Seq libraries for sequencing were constructed according to the NEXTflex ChIP-Seq library protocol outlined in NEXTflex ChIP-Seq Kit - 5143-01. Briefly, DNA was subjected to a series of enzymatic reactions that repair frayed ends, phosphorylate the fragments, add a single nucleotide A overhang and ligate adaptors (NEXTflex ChIP Barcodes-48 kit). The libraries are enriched using PCR (5 cycles), fragments were size selected using a 2 % Low melting agarose gel and purified using MinElute Gel Extraction Kit (QIAGEN). The libraries were further enriched using PCR (14 cycles), post PCR cleanup was performed using Agencourt AMPURE XP beads (Beckman Coulter #A63881). The prepared libraries were quantified using Qubit fluorometer and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS4594914 Nup93_ChIP seq Nup93_Input ChIP-Seq GENOMIC ChIP Illumina HiSeq 2500 SRX5649551 Nup93_ChIP_Replicate-1 SRP191222 bp0 Crosslinking 1% paraformaldehyde for 10 min, nuclear purification with 0.5% NP40, DNA solubilization and sonication in 1% SDS using Covaris sonicator, followed by Immunoprecipitation with following antibodies 1.Anti-Nup93 antibody (sc-292099)- 2g/ 100g of chromatin, 2. Normal Rabbit IgG (Millipore 12-370). Arpoximately 20-30 ng of DNA was used for library preparation. ChIP-Seq libraries for sequencing were constructed according to the NEXTflex ChIP-Seq library protocol outlined in NEXTflex ChIP-Seq Kit - 5143-01. Briefly, DNA was subjected to a series of enzymatic reactions that repair frayed ends, phosphorylate the fragments, add a single nucleotide A overhang and ligate adaptors (NEXTflex ChIP Barcodes-48 kit). The libraries are enriched using PCR (5 cycles), fragments were size selected using a 2 % Low melting agarose gel and purified using MinElute Gel Extraction Kit (QIAGEN). The libraries were further enriched using PCR (14 cycles), post PCR cleanup was performed using Agencourt AMPURE XP beads (Beckman Coulter #A63881). The prepared libraries were quantified using Qubit fluorometer and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS4594914 Nup93_ChIP seq Nup93_ChIP_Replicate-1 ChIP-Seq GENOMIC ChIP Illumina HiSeq 2500