SRX5652366 sm3 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597511 sm3, small RNA library, Sm9-III-2 biological replicate 1, Pinus sylvestris sm3 miRNA-Seq OTHER cDNA Ion Torrent PGM SRX5652367 sm4 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597513 sm4, small RNA library, Sm9-III-2 biological replicate 2, Pinus sylvestris sm4 miRNA-Seq OTHER cDNA Ion Torrent PGM SRX5652368 sm5 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597512 sm5, small RNA library, Sm9-III-2 biological replicate 3, Pinus sylvestris sm5 miRNA-Seq OTHER cDNA Ion Torrent PGM SRX5652369 sm6 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597514 sm6, small RNA library, Sm9-III-2 biological replicate 4, Pinus sylvestris sm6 miRNA-Seq OTHER cDNA Ion Torrent PGM SRX5652370 sm7 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597515 sm7, small RNA library, Sm9-III-2 biological replicate 5, Pinus sylvestris sm7 miRNA-Seq OTHER cDNA Ion Torrent PGM SRX5652371 sm8 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597516 sm8, small RNA library, Sm9-III-2 biological replicate 6, Pinus sylvestris sm8 miRNA-Seq OTHER cDNA Ion Torrent PGM SRX5652372 sm5-1 SRP191389 bp0 Total RNA was isolated from 100 mg of needles using a phenol:chloroform: isoamyl alcohol extraction protocol (Rubio-Pia and Prez, 2011). Total RNA and small RNA quality, quantity and integrity number (for total RNA) was verified using the Agilent Technologies 2100 Bioanalyzer with RNA Agilent RNA 6000 Nano Kit and Agilent Small RNA kit. Total RNA preparations were stored at -80 C. Total RNA samples were enriched for small RNA as outlined in the Ion RNA-Seq Library Preparation guide (#4476286 revision E) and 6 smallRNA non-barcoded libraries were prepared using Ion Total RNA-Seq Kit v2 for Small RNA Libraries (#4476289, Life Technologies) according to the manufacturers protocol. The amplified small RNA (cDNA non-barcoded) library quality and quantity were analysed using the Agilent Technologies 2100 Bioanalyzer with the High Sensitivity DNA Kit. Template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA were prepared with the Ion OneTouch 2 Instrument following the manufacturers protocol. Ion Sphere particle quality and quantity were assessed using the Qubit 2.0 Fluorometer and Ion Sphere Quality Control Kit, and then enriched by Ion OneTouch ES (Life Technologies) and according to the manufacturers protocol. Enriched template-positive ISPs were prepared and loaded onto an Ion 316 chip and sequenced on an Ion Personal Genome Machine (PGM) System at the Norwegian Institute ofBioeconomy Research (NIBIO). SRS4597518 sm5-1, small RNA library, Sm9-III-2 biological replicate 3, Pinus sylvestris sm5-1 miRNA-Seq OTHER cDNA Ion Torrent PGM