SRP194368 PRJNA540602 GSE130550 Role of nsd2 in Follicular helper T cells Follicular helper T cell (Tfh) provide essential help for humoral immune response. However, how Tfh differentiation is epigenetically regulated remains incompletely understood. Here we found that H3K36me2 methyltransferase Nsd2 is required for Tfh differentiation. Lack of Nsd2 leads to reduced Tfh generation and germinal center response. Mice with Nsd2 deficiency in T cells have reduced capability of chronic virus control. To understand the molecular mechanism by which Nsd2 regulates Tfh differentiation, RNA-seq analysis of Nsd2 wildtype and knockout Tfh cells were performed. Overall design: CD4+CXCR5+PD1+ Tfh cells were sorted from wildtype (WT) and CD4Cre+Nsd2fl/fl (KO) mice for RNA-seq analysis to identify the differential expressed genes. GSE130550 pubmed 31636135