SRX5796079 Bth GUIDE-Seq Bth/WT cell line SaCas9-KKH 4.2 gRNA SRP195383 PRJNA541170 GUIDE-Seq Bth/WT cell line SaCas9-KKH 4.2 gRNA: Y adapter: CGTACTAG, P adapter for plus library: CTCTCTAT, P adatper for minus library GTAAGGAG; GUIDE-Seq Bth/WT cell line SpCas9 2.4 gRNA: Y adapter: TAAGGCGA, P adapter for plus library: TAGATCGC, P adatper for minus library AGAGTAGA; GUIDE-Seq Bth/WT cell line oligo only: Y adapter: AGGCAGAA, P adapter for plus library: TATCCTCT, P adatper for minus library ACTGCATA SRS4727363 GUIDE-Seq analysis Bth OTHER GENOMIC other Illumina MiSeq