SRX5892137 Ahn.Mel04_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740266 SAMN11605815 Ahn.Mel04_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892138 Ahn.Mel01_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740247 SAMN11605812 Ahn.Mel01_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892139 Ahn.Mel13_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740243 SAMN11605824 Ahn.Mel13_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892140 Ahn.Mel10_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740248 SAMN11605821 Ahn.Mel10_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892141 Ahn.Mel26_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740265 SAMN11605837 Ahn.Mel26_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892142 Ahn.Mel19_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740258 SAMN11605830 Ahn.Mel19_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892143 Ahn.Mel32_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740233 SAMN11605843 Ahn.Mel32_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892144 Ahn.Mel28_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740237 SAMN11605839 Ahn.Mel28_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892145 Ahn.Mel35_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740235 SAMN11605845 Ahn.Mel35_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892146 Ahn.Mel34_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740236 SAMN11605844 Ahn.Mel34_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892147 Ahn.Mel38_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740231 SAMN11605847 Ahn.Mel38_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892148 Ahn.Mel37_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740232 SAMN11605846 Ahn.Mel37_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892149 Ahn.Mel43_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740270 SAMN11605850 Ahn.Mel43_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892150 Ahn.Mel42_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740269 SAMN11605849 Ahn.Mel42_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892151 Ahn.Mel46_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740260 SAMN11605853 Ahn.Mel46_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892152 Ahn.Mel44_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740262 SAMN11605851 Ahn.Mel44_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500 SRX5892153 Ahn.Mel47_MT SRP197281 PRJNA541981 Stool samples underwent shotgun metatranscriptome sequencing at the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory. RNA was extracted using the Mo Bio PowerMicrobiome RNA Isolation Kit, and quantified using a Qubit Fluorometer. RNA integrity and size distribution were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer. Samples then underwent DNase treatment using the Turbo DNA-free kit (Life Technologies), and ribosomal depletion using the Ribo-Zero rRNA Removal Kit (Bacteria) (Illumina). Bacterial mRNA purification was achieved with AMPure RNAClean XP Beads, and cDNA libraries were generated using the ScriptSeq V2 RNA-Seq Library Preparation Kit (Illumina). Libraries were sequenced on the Illumina HiSeq 2500 on a 2X151bp paired end run. In this study, metatranscriptomic library preparation failed for 10 samples due to poor RNA quality; thus only a subset of 17 patient samples underwent metatranscriptomic sequencing. SRS4740261 SAMN11605854 Ahn.Mel47_MT RNA-Seq METATRANSCRIPTOMIC RANDOM Illumina HiSeq 2500