RNA-seq of Arabidopsis seedlings: wild-type treated with NaCl for 36 hours

SRX5904183 WTN36 RNA-seq of Arabidopsis seedlings: wild-type treated with NaCl for 36 hours SRP199586 bp0 mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end. SRS4824291 SAMN10830047 WTN36 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000 SRX5904184 os1_4N36 RNA-seq of Arabidopsis seedlings: T-DNA insertion mutation treated with NaCl for 36 hours SRP199586 bp0 mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end. SRS4824292 SAMN10830048 os1_4N36 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000 SRX5904185 WT RNA-seq of Arabidopsis seedlings: wild-type SRP199586 bp0 mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end. SRS4824293 SAMN10830043 WT RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000 SRX5904186 os1_4 RNA-seq of Arabidopsis seedlings: T-DNA insertion mutation SRP199586 bp0 mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end. SRS4824294 SAMN10830044 os1_4 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000 SRX5904187 WTS36 RNA-seq of Arabidopsis seedlings: wild-type treated with sorbitol for 36 hours SRP199586 bp0 mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end. SRS4824295 SAMN10830045 WTS36 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000 SRX5904188 os1_4S36 RNA-seq of Arabidopsis seedlings: T-DNA insertion mutation treated with sorbitol for 36 hours SRP199586 bp0 mRNA was enriched through A-T complementary pairing using Oligo (dT) beads, and then mRNA was broke into short pieces in fragmentation buffer. The mRNA fragments were used as templates to synthesis cDNA using random hexamers followed by purification using AMPure XP beads. After purification, the double-strand cDNA underwent the end repair and connection of sequencing adapter, then was enriched by PCR to obtain the cDNA library in the end. SRS4824296 SAMN10830046 os1_4S36 RNA-Seq TRANSCRIPTOMIC RANDOM PCR Illumina HiSeq 2000