SRX6073768 GSM3892047 SRP201506 SRS4975929 GSM3892047 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892047 GSM3892047 GEO Accession GSM3892047 SRX6073769 GSM3892048 SRP201506 SRS4975930 GSM3892048 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892048 GSM3892048 GEO Accession GSM3892048 SRX6073770 GSM3892049 SRP201506 SRS4975931 GSM3892049 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892049 GSM3892049 GEO Accession GSM3892049 SRX6073771 GSM3892050 SRP201506 SRS4975932 GSM3892050 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892050 GSM3892050 GEO Accession GSM3892050 SRX6073772 GSM3892051 SRP201506 SRS4975933 GSM3892051 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892051 GSM3892051 GEO Accession GSM3892051 SRX6073773 GSM3892052 SRP201506 SRS4975934 GSM3892052 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892052 GSM3892052 GEO Accession GSM3892052 SRX6073774 GSM3892053 SRP201506 SRS4975935 GSM3892053 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892053 GSM3892053 GEO Accession GSM3892053 SRX6073775 GSM3892054 SRP201506 SRS4975936 GSM3892054 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892054 GSM3892054 GEO Accession GSM3892054 SRX6073776 GSM3892046 SRP201506 SRS4975937 GSM3892046 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892046 GSM3892046 GEO Accession GSM3892046 SRX6073777 GSM3892063 SRP201506 SRS4975938 GSM3892063 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892063 GSM3892063 GEO Accession GSM3892063 SRX6073778 GSM3892064 SRP201506 SRS4975939 GSM3892064 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892064 GSM3892064 GEO Accession GSM3892064 SRX6073779 GSM3892065 SRP201506 SRS4975940 GSM3892065 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892065 GSM3892065 GEO Accession GSM3892065 SRX6073780 GSM3892066 SRP201506 SRS4975941 GSM3892066 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892066 GSM3892066 GEO Accession GSM3892066 SRX6073781 GSM3892067 SRP201506 SRS4975942 GSM3892067 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892067 GSM3892067 GEO Accession GSM3892067 SRX6073782 GSM3892068 SRP201506 SRS4975943 GSM3892068 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892068 GSM3892068 GEO Accession GSM3892068 SRX6073783 GSM3892069 SRP201506 SRS4975944 GSM3892069 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892069 GSM3892069 GEO Accession GSM3892069 SRX6073784 GSM3892070 SRP201506 SRS4975945 GSM3892070 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892070 GSM3892070 GEO Accession GSM3892070 SRX6073785 GSM3892119 SRP201506 SRS4975946 GSM3892119 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892119 GSM3892119 GEO Accession GSM3892119 SRX6073786 GSM3892120 SRP201506 SRS4975947 GSM3892120 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892120 GSM3892120 GEO Accession GSM3892120 SRX6073787 GSM3892087 SRP201506 SRS4975948 GSM3892087 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892087 GSM3892087 GEO Accession GSM3892087 SRX6073788 GSM3892088 SRP201506 SRS4975949 GSM3892088 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892088 GSM3892088 GEO Accession GSM3892088 SRX6073789 GSM3892089 SRP201506 SRS4975950 GSM3892089 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892089 GSM3892089 GEO Accession GSM3892089 SRX6073790 GSM3892090 SRP201506 SRS4975951 GSM3892090 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892090 GSM3892090 GEO Accession GSM3892090 SRX6073791 GSM3892091 SRP201506 SRS4975952 GSM3892091 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892091 GSM3892091 GEO Accession GSM3892091 SRX6073792 GSM3892092 SRP201506 SRS4975953 GSM3892092 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892092 GSM3892092 GEO Accession GSM3892092 SRX6073793 GSM3892093 SRP201506 SRS4975954 GSM3892093 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892093 GSM3892093 GEO Accession GSM3892093 SRX6073794 GSM3892094 SRP201506 SRS4975955 GSM3892094 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892094 GSM3892094 GEO Accession GSM3892094 SRX6073795 GSM3892111 SRP201506 SRS4975956 GSM3892111 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892111 GSM3892111 GEO Accession GSM3892111 SRX6073796 GSM3892112 SRP201506 SRS4975957 GSM3892112 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892112 GSM3892112 GEO Accession GSM3892112 SRX6073797 GSM3892113 SRP201506 SRS4975958 GSM3892113 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892113 GSM3892113 GEO Accession GSM3892113 SRX6073798 GSM3892114 SRP201506 SRS4975959 GSM3892114 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892114 GSM3892114 GEO Accession GSM3892114 SRX6073799 GSM3892115 SRP201506 SRS4975960 GSM3892115 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892115 GSM3892115 GEO Accession GSM3892115 SRX6073800 GSM3892116 SRP201506 SRS4975961 GSM3892116 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892116 GSM3892116 GEO Accession GSM3892116 SRX6073801 GSM3892117 SRP201506 SRS4975962 GSM3892117 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892117 GSM3892117 GEO Accession GSM3892117 SRX6073802 GSM3892118 SRP201506 SRS4975963 GSM3892118 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892118 GSM3892118 GEO Accession GSM3892118 SRX6073803 GSM3892055 SRP201506 SRS4975964 GSM3892055 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892055 GSM3892055 GEO Accession GSM3892055 SRX6073804 GSM3892056 SRP201506 SRS4975965 GSM3892056 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892056 GSM3892056 GEO Accession GSM3892056 SRX6073805 GSM3892057 SRP201506 SRS4975966 GSM3892057 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892057 GSM3892057 GEO Accession GSM3892057 SRX6073806 GSM3892058 SRP201506 SRS4975967 GSM3892058 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892058 GSM3892058 GEO Accession GSM3892058 SRX6073807 GSM3892059 SRP201506 SRS4975968 GSM3892059 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892059 GSM3892059 GEO Accession GSM3892059 SRX6073808 GSM3892060 SRP201506 SRS4975969 GSM3892060 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892060 GSM3892060 GEO Accession GSM3892060 SRX6073809 GSM3892061 SRP201506 SRS4975970 GSM3892061 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892061 GSM3892061 GEO Accession GSM3892061 SRX6073810 GSM3892062 SRP201506 SRS4975971 GSM3892062 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892062 GSM3892062 GEO Accession GSM3892062 SRX6073811 GSM3892079 SRP201506 SRS4975972 GSM3892079 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892079 GSM3892079 GEO Accession GSM3892079 SRX6073812 GSM3892080 SRP201506 SRS4975973 GSM3892080 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892080 GSM3892080 GEO Accession GSM3892080 SRX6073813 GSM3892081 SRP201506 SRS4975974 GSM3892081 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892081 GSM3892081 GEO Accession GSM3892081 SRX6073814 GSM3892082 SRP201506 SRS4975975 GSM3892082 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892082 GSM3892082 GEO Accession GSM3892082 SRX6073815 GSM3892083 SRP201506 SRS4975976 GSM3892083 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892083 GSM3892083 GEO Accession GSM3892083 SRX6073816 GSM3892084 SRP201506 SRS4975977 GSM3892084 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892084 GSM3892084 GEO Accession GSM3892084 SRX6073817 GSM3892085 SRP201506 SRS4975978 GSM3892085 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892085 GSM3892085 GEO Accession GSM3892085 SRX6073818 GSM3892086 SRP201506 SRS4975979 GSM3892086 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892086 GSM3892086 GEO Accession GSM3892086 SRX6073819 GSM3892103 SRP201506 SRS4975980 GSM3892103 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892103 GSM3892103 GEO Accession GSM3892103 SRX6073820 GSM3892104 SRP201506 SRS4975981 GSM3892104 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892104 GSM3892104 GEO Accession GSM3892104 SRX6073821 GSM3892105 SRP201506 SRS4975982 GSM3892105 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892105 GSM3892105 GEO Accession GSM3892105 SRX6073822 GSM3892106 SRP201506 SRS4975983 GSM3892106 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892106 GSM3892106 GEO Accession GSM3892106 SRX6073823 GSM3892107 SRP201506 SRS4975984 GSM3892107 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892107 GSM3892107 GEO Accession GSM3892107 SRX6073824 GSM3892108 SRP201506 SRS4975985 GSM3892108 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892108 GSM3892108 GEO Accession GSM3892108 SRX6073825 GSM3892109 SRP201506 SRS4975986 GSM3892109 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892109 GSM3892109 GEO Accession GSM3892109 SRX6073826 GSM3892110 SRP201506 SRS4975987 GSM3892110 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892110 GSM3892110 GEO Accession GSM3892110 SRX6073827 GSM3892071 SRP201506 SRS4975988 GSM3892071 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892071 GSM3892071 GEO Accession GSM3892071 SRX6073828 GSM3892072 SRP201506 SRS4975989 GSM3892072 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892072 GSM3892072 GEO Accession GSM3892072 SRX6073829 GSM3892073 SRP201506 SRS4975990 GSM3892073 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892073 GSM3892073 GEO Accession GSM3892073 SRX6073830 GSM3892074 SRP201506 SRS4975991 GSM3892074 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892074 GSM3892074 GEO Accession GSM3892074 SRX6073831 GSM3892075 SRP201506 SRS4975992 GSM3892075 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892075 GSM3892075 GEO Accession GSM3892075 SRX6073832 GSM3892076 SRP201506 SRS4975993 GSM3892076 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892076 GSM3892076 GEO Accession GSM3892076 SRX6073833 GSM3892077 SRP201506 SRS4975994 GSM3892077 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892077 GSM3892077 GEO Accession GSM3892077 SRX6073834 GSM3892078 SRP201506 SRS4975995 GSM3892078 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892078 GSM3892078 GEO Accession GSM3892078 SRX6073835 GSM3892095 SRP201506 SRS4975996 GSM3892095 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892095 GSM3892095 GEO Accession GSM3892095 SRX6073836 GSM3892096 SRP201506 SRS4975997 GSM3892096 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892096 GSM3892096 GEO Accession GSM3892096 SRX6073837 GSM3892097 SRP201506 SRS4975998 GSM3892097 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892097 GSM3892097 GEO Accession GSM3892097 SRX6073838 GSM3892098 SRP201506 SRS4975999 GSM3892098 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892098 GSM3892098 GEO Accession GSM3892098 SRX6073839 GSM3892099 SRP201506 SRS4976000 GSM3892099 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892099 GSM3892099 GEO Accession GSM3892099 SRX6073840 GSM3892100 SRP201506 SRS4976001 GSM3892100 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892100 GSM3892100 GEO Accession GSM3892100 SRX6073841 GSM3892101 SRP201506 SRS4976002 GSM3892101 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892101 GSM3892101 GEO Accession GSM3892101 SRX6073842 GSM3892102 SRP201506 SRS4976003 GSM3892102 OTHER GENOMIC other Total genomic DNA was extracted with the Quick-gDNA MiniPrep Kit (Zymo Research Corporation) according to manufacturer's instructions. Synthetic Lethal Screen : 4x10^6 cells from each of the cell lines (UMSCC8, UMSCC25, HN12, Cal27, JHU011) were plated into duplicate 15cm plates (3x10^6 cells/plate) and transduced 24 hours later with the kinome shRNA library containing viral supernatant and 8 μg/ml polybrene (Sigma-Aldrich). After 72 hours, transfected cells were selected with puromycin (1 μg/ml) for 5 days. The lentiviral preparation for the SBI genome-wide screen was reported in our previous publication Essential Kinome Screen : Target cell lines (UMSCC8, UMSCC25, HN6, HN12, Cal27, JHU011) were plated at 5x10^6 cells per 15 cm plate, 2 plates per cell line, in 20 mL growth medium. The following day, cells were pretreated with 1 µg/mL polybrene for 30 minutes. The media containing the packaged lentiviral kinome shRNA library was added to each plate of target cells at a MOI of ~0.2, incubated for 16 hours and then removed and replaced with fresh growth medium (lacking puromycin). Forty-eight hours post-infection, each cell line was trypsinized and plated at a density of at least 2x10^6 cells per 15 cm plate in quadruplicate. Additionally, at the same 6 time point, four replicates of 4x10^6 cells each from each cell line were pelleted. Illumina Genome Analyzer IIx gds 303892102 GSM3892102 GEO Accession GSM3892102