SRX6075217 GSM3892300 SRP201545 SRS4977303 GSM3892300 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892300 GSM3892300 GEO Accession GSM3892300 SRX6075218 GSM3892301 SRP201545 SRS4977304 GSM3892301 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892301 GSM3892301 GEO Accession GSM3892301 SRX6075219 GSM3892302 SRP201545 SRS4977305 GSM3892302 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892302 GSM3892302 GEO Accession GSM3892302 SRX6075220 GSM3892303 SRP201545 SRS4977306 GSM3892303 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892303 GSM3892303 GEO Accession GSM3892303 SRX6075221 GSM3892304 SRP201545 SRS4977307 GSM3892304 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892304 GSM3892304 GEO Accession GSM3892304 SRX6075222 GSM3892305 SRP201545 SRS4977308 GSM3892305 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892305 GSM3892305 GEO Accession GSM3892305 SRX6075223 GSM3892306 SRP201545 SRS4977309 GSM3892306 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892306 GSM3892306 GEO Accession GSM3892306 SRX6075224 GSM3892307 SRP201545 SRS4977310 GSM3892307 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892307 GSM3892307 GEO Accession GSM3892307 SRX6075225 GSM3892308 SRP201545 SRS4977311 GSM3892308 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892308 GSM3892308 GEO Accession GSM3892308 SRX6075226 GSM3892309 SRP201545 SRS4977312 GSM3892309 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892309 GSM3892309 GEO Accession GSM3892309 SRX6075227 GSM3892310 SRP201545 SRS4977313 GSM3892310 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892310 GSM3892310 GEO Accession GSM3892310 SRX6075228 GSM3892311 SRP201545 SRS4977314 GSM3892311 RNA-Seq TRANSCRIPTOMIC cDNA Aproximately 1000 mid-L4 stage animals were washed, resuspended in QIAzol lysis reagent (Qiagen, Venlo, Netherlands, #79306), snap frozen in liquid nitrogen, and stored in -80°C freezer until RNA extraction. To break the worm cuticle and improve RNA yield, all samples underwent 5 freeze-thaw cycles. In each cycle, samples were thawed at 37˚C and then snap-frozen in liquid nitrogen. RNA extraction was performed immediately using QIAGEN RNeasy Mini Kit (QIAGEN, #74104) following manufacturer's instructions. RNA quality was confirmed using Agilent Bioanalyzer 2100 (Agilent Technologies). All samples passed the quality control standard of RIN>8.0. mRNA libraries were prepared using TruSeq® Stranded mRNA LT - Set A kit (Illumina, CA, USA RS-122-2101) following manufacturer's instructions and linked to different adaptors to enable pooling. Library quality was checked using 2200 High Sensitivity D1000 Tape Station (Agilent Technologies) RNA-Seq with Illumina HiSeq 2500 using 50-cycle, pair-end settings Illumina HiSeq 2500 gds 303892311 GSM3892311 GEO Accession GSM3892311