SRX6098142 GSM3899392 SRP201985 SRS4998678 GSM3899392 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899392 GSM3899392 GEO Accession GSM3899392 SRX6098143 GSM3899393 SRP201985 SRS4998677 GSM3899393 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899393 GSM3899393 GEO Accession GSM3899393 SRX6098144 GSM3899394 SRP201985 SRS4998679 GSM3899394 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899394 GSM3899394 GEO Accession GSM3899394 SRX6098145 GSM3899395 SRP201985 SRS4998680 GSM3899395 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899395 GSM3899395 GEO Accession GSM3899395 SRX6098146 GSM3899396 SRP201985 SRS4998681 GSM3899396 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899396 GSM3899396 GEO Accession GSM3899396 SRX6098147 GSM3899397 SRP201985 SRS4998682 GSM3899397 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899397 GSM3899397 GEO Accession GSM3899397 SRX6098148 GSM3899398 SRP201985 SRS4998683 GSM3899398 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899398 GSM3899398 GEO Accession GSM3899398 SRX6098149 GSM3899399 SRP201985 SRS4998684 GSM3899399 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899399 GSM3899399 GEO Accession GSM3899399 SRX6098150 GSM3899400 SRP201985 SRS4998685 GSM3899400 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899400 GSM3899400 GEO Accession GSM3899400 SRX6098151 GSM3899401 SRP201985 SRS4998686 GSM3899401 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitations were performed as described previously, with adaptations (Schmidt et al., 2009). In short, cells were crosslinked in solution A (pH 7.4, 50mM Hepes, 100mM NaCl, 1mM EDTA, 0.5M EGTA) containing 2mM DSG for 35 minutes, then formaldehyde was added to a final concentration of 1% and incubated for another 10 minutes. After addition of Glycine to a final concentration of 125mM to quench the crosslinking reaction and washing with PBS, cells were collected. The Bioruptor Pico (Diagenode) was used for sonication. For ChIP, antibodies were used to detect TRPS1 (10ug, sc-26974, Santa Cruz; 5ug, AF4838, R&D) and GATA3 (10ug, sc-268, Santa Cruz) with 100 ml Protein A or G magnetic beads (Thermo Scientific). Immunoprecipitated DNA was processed for library preparation (Part# 0801-0303, KAPA biosystems kit). Illumina HiSeq 2500 gds 303899401 GSM3899401 GEO Accession GSM3899401