<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT alias="GSM3911794" accession="SRX6384670">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX6384670</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3911794</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3911794: SingleNuclei_nextseq1; Homo sapiens; Canis lupus familiaris; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP212648" refname="GSE133546">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP212648</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS5044188">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS5044188</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM3911794</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>PBMCs were isolated according to the manufacturer's instructions for FICOLL extraction (pluriSelect).Minced mouse colon was digested in 1X HBSS and 83 µl of collagenase IV (final concentration 166 U/ml) for 15 min at 37ºC and filtered through a 70 µm nylon mesh. Sequencing librraries were prepared according to the respective single-cell RNA-seq protocols as detailed in the Methods and referenced in TableS1. Library selection and sequencing was performed following recommendations of to the respective single-cell RNA-seq protocols as detailed in the Methods.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303911794</ID>
          <LABEL>GSM3911794</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
  </EXPERIMENT>
  <EXPERIMENT alias="GSM3911795" accession="SRX6384671">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX6384671</PRIMARY_ID>
      <SUBMITTER_ID namespace="GEO">GSM3911795</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>GSM3911795: SingleNuclei_nextseq2; Homo sapiens; Canis lupus familiaris; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP212648" refname="GSE133546">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP212648</PRIMARY_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS5044189">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS5044189</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM3911795</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <PAIRED/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>PBMCs were isolated according to the manufacturer's instructions for FICOLL extraction (pluriSelect).Minced mouse colon was digested in 1X HBSS and 83 µl of collagenase IV (final concentration 166 U/ml) for 15 min at 37ºC and filtered through a 70 µm nylon mesh. Sequencing librraries were prepared according to the respective single-cell RNA-seq protocols as detailed in the Methods and referenced in TableS1. Library selection and sequencing was performed following recommendations of to the respective single-cell RNA-seq protocols as detailed in the Methods.</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
    <EXPERIMENT_LINKS>
      <EXPERIMENT_LINK>
        <XREF_LINK>
          <DB>gds</DB>
          <ID>303911795</ID>
          <LABEL>GSM3911795</LABEL>
        </XREF_LINK>
      </EXPERIMENT_LINK>
    </EXPERIMENT_LINKS>
  </EXPERIMENT>
</EXPERIMENT_SET>
