SRX6589624 GSM3972786 SRP216384 SRS5155017 GSM3972786 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972786 GSM3972786 GEO Accession GSM3972786 SRX6589625 GSM3972787 SRP216384 SRS5155018 GSM3972787 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972787 GSM3972787 GEO Accession GSM3972787 SRX6589626 GSM3972788 SRP216384 SRS5155019 GSM3972788 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972788 GSM3972788 GEO Accession GSM3972788 SRX6589627 GSM3972789 SRP216384 SRS5155020 GSM3972789 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972789 GSM3972789 GEO Accession GSM3972789 SRX6589628 GSM3972790 SRP216384 SRS5155021 GSM3972790 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972790 GSM3972790 GEO Accession GSM3972790 SRX6589629 GSM3972791 SRP216384 SRS5155022 GSM3972791 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972791 GSM3972791 GEO Accession GSM3972791 SRX6589630 GSM3972792 SRP216384 SRS5155023 GSM3972792 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972792 GSM3972792 GEO Accession GSM3972792 SRX6589631 GSM3972793 SRP216384 SRS5155024 GSM3972793 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972793 GSM3972793 GEO Accession GSM3972793 SRX6589632 GSM3972794 SRP216384 SRS5155025 GSM3972794 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972794 GSM3972794 GEO Accession GSM3972794 SRX6589633 GSM3972795 SRP216384 SRS5155026 GSM3972795 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972795 GSM3972795 GEO Accession GSM3972795 SRX6589634 GSM3972796 SRP216384 SRS5155027 GSM3972796 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972796 GSM3972796 GEO Accession GSM3972796 SRX6589635 GSM3972797 SRP216384 SRS5155028 GSM3972797 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972797 GSM3972797 GEO Accession GSM3972797 SRX6589636 GSM3972798 SRP216384 SRS5155030 GSM3972798 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972798 GSM3972798 GEO Accession GSM3972798 SRX6589637 GSM3972799 SRP216384 SRS5155029 GSM3972799 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972799 GSM3972799 GEO Accession GSM3972799 SRX6589638 GSM3972800 SRP216384 SRS5155031 GSM3972800 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972800 GSM3972800 GEO Accession GSM3972800 SRX6589639 GSM3972801 SRP216384 SRS5155032 GSM3972801 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972801 GSM3972801 GEO Accession GSM3972801 SRX6589640 GSM3972802 SRP216384 SRS5155033 GSM3972802 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972802 GSM3972802 GEO Accession GSM3972802 SRX6589641 GSM3972803 SRP216384 SRS5155034 GSM3972803 RNA-Seq TRANSCRIPTOMIC cDNA Cells were washed twice with cold PBS and lysed in 6 mL of lysis buffer (20 mM MOPS-KOH [pH 7.4], 120 mM KCl, 0.5% Igepal, 2 mM b-mercaptoethanol), supplemented with 200 U/mL RNasin (Promega) and Complete Protease Inhibitor Cocktail (Roche). Cell lysates were incubated on ice for 20 min, with gentle mixing every 5 min, and then clarified by centrifugation at 16,0003g for 15 min at 4°C. A 250-mL aliquot of each cell lysate was used to perform total RNA purification using TRI Reagent LS [Sigma]). The remaining cell lysate was incubated for 2 hr on a spinning wheel at 4°C with 200 mL StrepTactin Sepharose High Performance beads (GE Healthcare). Beads were collected by centrifugation (1,600 g) for 5 min at 4°C) and washed twice for 5 min on a spinning wheel with 5 mL washing buffer (20mMMOPS-KOH [pH 7.4], 120mMKCl, 2mMb-mercaptoethanol) supplemented with 200 U/mL RNasin and complete protease inhibitor cocktail. Precipitates were eluted using biotin elution buffer (IBA Lifesciences). RNA purification was performed using TRI Reagent LS (T3934; Sigma). RNA was dissolved in 50 mL DNase-free and RNase-free ultrapure water. Extracted RNAs were analyzed using Nanovue (GE Healthcare) and the Bioanalyser RNA nano kit (Agilent Technologies). For library preparation TruSeq stranded total RNA library prep kit (#20020596, Illumina) was used. NGS libraries were generated following the manufacturer's protocol. The indexed samples were multiplexed per 4 and sequenced on a Illumina Hiseq 2500 sequencer (Illumina) to produce single-ends 65 bases reads, bearing strand specificity. Illumina HiSeq 2500 gds 303972803 GSM3972803 GEO Accession GSM3972803