SRP216454 PRJNA556762 P. aeruginosa Houston-1 genome Hybrid de novo assembly of Illumina/Nanopore sequence data produced complete circular sequences of the chromosome and a plasmid for the multidrug-resistant Pseudomonas aeruginosa Houston-1 strain. This provides a high-quality representative sequence for a lineage endemic within a pediatric cystic fibrosis care center at Texas Children's Hospital.