SRP218676 PRJNA560672 RAD sequencing of C. niger workers The genus Cataglyphis presents a high level of diversification, making it an excellent model with which to study evolutionary paths. Israel appears to be a “hot spot” for recent speciation in this genus. Although previous studies have described multiple species of Cataglyphis in Israel, a recent genetic study has questioned the existence of some of these historically described species. The present study focuses on an apparent species complex, the C. niger species complex which includes C. niger, C. savigyi, and C. drusus that are distinguishable by their mitochondrial DNA (and therefore named mitotypes). However, as was shown by this Restriction site associated DNA sequencing (RAD-seq) of 70 individuals in the complex, they are indistinguishable by their nuclear DNA.