SRX6810757 LIB30104_R1_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354718 Col-0_control_22C LIB30104_R1_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810758 LIB30104_R1_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354718 Col-0_control_22C LIB30104_R1_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810759 LIB30106_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354719 Col-0_control_4C LIB30106_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810760 LIB30107_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354719 Col-0_control_4C LIB30107_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810761 LIB30108_R1_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354720 Col-0_AzadC_22C LIB30108_R1_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810762 LIB30108_R1_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354720 Col-0_AzadC_22C LIB30108_R1_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810763 LIB30109_R2_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354720 Col-0_AzadC_22C LIB30109_R2_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810764 LIB30109_R2_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354720 Col-0_AzadC_22C LIB30109_R2_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810765 LIB30108_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354720 Col-0_AzadC_22C LIB30108_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810766 LIB30109_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354720 Col-0_AzadC_22C LIB30109_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810767 LIB30110_R1_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354721 Col-0_AzadC_4C LIB30110_R1_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810768 LIB30110_R1_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354721 Col-0_AzadC_4C LIB30110_R1_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810769 LIB30105_R2_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354718 Col-0_control_22C LIB30105_R2_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810770 LIB30111_R2_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354721 Col-0_AzadC_4C LIB30111_R2_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810771 LIB30111_R2_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354721 Col-0_AzadC_4C LIB30111_R2_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810772 LIB30110_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354721 Col-0_AzadC_4C LIB30110_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810773 LIB30111_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354721 Col-0_AzadC_4C LIB30111_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810774 LIB30105_R2_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354718 Col-0_control_22C LIB30105_R2_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810775 LIB30104_R3_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354718 Col-0_control_22C LIB30104_R3_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810776 LIB30105_R3_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354718 Col-0_control_22C LIB30105_R3_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810777 LIB30106_R1_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354719 Col-0_control_4C LIB30106_R1_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810778 LIB30106_R1_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354719 Col-0_control_4C LIB30106_R1_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810779 LIB30107_R2_L7 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354719 Col-0_control_4C LIB30107_R2_L7 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX6810780 LIB30107_R2_L8 SRP220616 bp0 libraries were constructed for 12 RNA samples using the TruSeq RNA protocol (Illumina 15026495 Rev.F). The library preparation involved an initial QC of the RNA using Qubit DNA (Life technologies Q32854) and RNA (Life technologies Q32852) assays as well as a quality check using the Bioanalyser with the Nano kit (Agilent 5067-1511). One g of this RNA was used to obtain mRNA with a poly- A pull down using biotin beads, fragmented at 94C for 6 minutes and random hexamers were used for first-strand cDNA synthesis. The 78 libraries had an average insert size of 270 bp. The insert size of the libraries was verified by running an aliquot of the library on the Agilent bioanalyser using the High Sensitivity chip (Agilent 5067-4626) and the concentration was determined by using a High Sensitivity Qubit assay (ThermoFisher Q32854). A 16-plex equimolar pool was prepared and checked by q-PCR, before preparing and loading for sequencing on the Hiseq 4000 (Illumina) using 150 paired-end reads across 3 lanes. The total number of raw reads generated in the RNA-seq data was ~ 23 M per biological replicate for each sample. SRS5354719 Col-0_control_4C LIB30107_R2_L8 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000