SRX6813434 GSM4066068 SRP220694 SRS5357308 GSM4066068 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066068 GSM4066068 GEO Accession GSM4066068 SRX6813435 GSM4066069 SRP220694 SRS5357309 GSM4066069 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066069 GSM4066069 GEO Accession GSM4066069 SRX6813436 GSM4066070 SRP220694 SRS5357310 GSM4066070 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066070 GSM4066070 GEO Accession GSM4066070 SRX6813437 GSM4066071 SRP220694 SRS5357311 GSM4066071 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066071 GSM4066071 GEO Accession GSM4066071 SRX6813438 GSM4066072 SRP220694 SRS5357312 GSM4066072 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066072 GSM4066072 GEO Accession GSM4066072 SRX6813439 GSM4066073 SRP220694 SRS5357313 GSM4066073 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066073 GSM4066073 GEO Accession GSM4066073 SRX6813440 GSM4066074 SRP220694 SRS5357314 GSM4066074 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066074 GSM4066074 GEO Accession GSM4066074 SRX6813441 GSM4066075 SRP220694 SRS5357315 GSM4066075 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066075 GSM4066075 GEO Accession GSM4066075 SRX6813442 GSM4066076 SRP220694 SRS5357316 GSM4066076 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066076 GSM4066076 GEO Accession GSM4066076 SRX6813443 GSM4066077 SRP220694 SRS5357317 GSM4066077 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066077 GSM4066077 GEO Accession GSM4066077 SRX6813444 GSM4066078 SRP220694 SRS5357318 GSM4066078 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066078 GSM4066078 GEO Accession GSM4066078 SRX6813445 GSM4066079 SRP220694 SRS5357319 GSM4066079 RNA-Seq TRANSCRIPTOMIC cDNA Cellular RNA was isolated using the NucleoSpin® RNA Isolation kit (Macherey-Nagel, Düren, Germany) according to manufacturer's instructions. For each experimental condition, three biological replicates were analyzed. After initial quality control using Agilent's Bioanalyzer, sequencing libraries were prepared from 500ng of total RNA per sample following Roche's stranded “KAPA RNA HyperPrep” library preparation protocol for single indexed Illumina libraries: First the polyA-RNA fraction was enriched using oligo-dT-probed paramagnetic beads. Enriched RNA was heat-fragmented and subjected to first strand synthesis using random priming. The second strand was synthesized incorporating dUTP instead of dTTP to preserve strand information. After A-tailing Illumina sequencing compatible adapters were ligated. Following bead-based clean-up steps the libraries were amplified using 10 cycles of PCR. Library quality and size was checked with qBit, Agilent Bioanalyzer and qPCR. Sequencing was carried out on an Illumina HiSeq 4000 system in PE75bp mode yielding between 15-23 million fragments per sample. Illumina HiSeq 4000 gds 304066079 GSM4066079 GEO Accession GSM4066079 SRX9808611 GSM5011994 SRP220694 PRJNA564311 SRS7992644 GSM5011994 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde and quenched by adding glycine 125 mM for 5 min at 4 °C. After this, cells were harvested and lysed according to the protocol described by Lee et al. and chromatin was sheared by sonication using the Bioruptor ultrasonicator (Diagenode). Sheared chromatin was immunoprecipitated using 10 μg of total SMAD1 antibody (Cell Signaling, #9743) or the normal rabbit IgG antibody (Cell Signaling, #2729) as a control, followed by incubation with protein G magnetic beads (Invitrogen). Eluted DNA was purified using DNA purification buffers and spin columns kit, according to the manufacturer's instructions (Cell Signaling). NEBNext Ultra II DNA library preparation kit was used according to manufacturer's instruction. HiSeq X Ten gds 305011994 GSM5011994 GEO Accession GSM5011994 SRX9808612 GSM5011995 SRP220694 PRJNA564311 SRS7992645 GSM5011995 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde and quenched by adding glycine 125 mM for 5 min at 4 °C. After this, cells were harvested and lysed according to the protocol described by Lee et al. and chromatin was sheared by sonication using the Bioruptor ultrasonicator (Diagenode). Sheared chromatin was immunoprecipitated using 10 μg of total SMAD1 antibody (Cell Signaling, #9743) or the normal rabbit IgG antibody (Cell Signaling, #2729) as a control, followed by incubation with protein G magnetic beads (Invitrogen). Eluted DNA was purified using DNA purification buffers and spin columns kit, according to the manufacturer's instructions (Cell Signaling). NEBNext Ultra II DNA library preparation kit was used according to manufacturer's instruction. HiSeq X Ten gds 305011995 GSM5011995 GEO Accession GSM5011995 SRX9808613 GSM5011996 SRP220694 PRJNA564311 SRS7992646 GSM5011996 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde and quenched by adding glycine 125 mM for 5 min at 4 °C. After this, cells were harvested and lysed according to the protocol described by Lee et al. and chromatin was sheared by sonication using the Bioruptor ultrasonicator (Diagenode). Sheared chromatin was immunoprecipitated using 10 μg of total SMAD1 antibody (Cell Signaling, #9743) or the normal rabbit IgG antibody (Cell Signaling, #2729) as a control, followed by incubation with protein G magnetic beads (Invitrogen). Eluted DNA was purified using DNA purification buffers and spin columns kit, according to the manufacturer's instructions (Cell Signaling). NEBNext Ultra II DNA library preparation kit was used according to manufacturer's instruction. HiSeq X Ten gds 305011996 GSM5011996 GEO Accession GSM5011996 SRX9808614 GSM5011997 SRP220694 PRJNA564311 SRS7992647 GSM5011997 ChIP-Seq GENOMIC ChIP Cells were fixed in 1% formaldehyde and quenched by adding glycine 125 mM for 5 min at 4 °C. After this, cells were harvested and lysed according to the protocol described by Lee et al. and chromatin was sheared by sonication using the Bioruptor ultrasonicator (Diagenode). Sheared chromatin was immunoprecipitated using 10 μg of total SMAD1 antibody (Cell Signaling, #9743) or the normal rabbit IgG antibody (Cell Signaling, #2729) as a control, followed by incubation with protein G magnetic beads (Invitrogen). Eluted DNA was purified using DNA purification buffers and spin columns kit, according to the manufacturer's instructions (Cell Signaling). NEBNext Ultra II DNA library preparation kit was used according to manufacturer's instruction. HiSeq X Ten gds 305011997 GSM5011997 GEO Accession GSM5011997