RNA-Seq of Stage 11 Aquilegia stamen

SRX6849018 AqStamen_pre RNA-Seq of Stage 11 Aquilegia stamen SRP221616 bp0 Total RNA was isolated using the Qiagen RNeasy Plant Mini kit (Qiagen, Valencia, CA, USA), including a repeated elution step. RNA quality was analyzed using a NanoDrop 8000 spectrophotometer (Thermofisher) and an Agilent Bioanalyzer. RNA samples with a minimum 2.09 260/280, and RNA integrity number of 7.5 were used as input using the Apollo Prep X PolyA8 mRNA 200 bp beta V1 protocol for single-end library generation. All libraries were quality confirmed for correct size distributions by TapeStation, quantified by QBIT and quantitative PCR using the ABI prism 7900 and Kappa quantification kit and pooled before running on one Illumina HiSeq2500 lane. SRS5387479 Stamen Pre AqStamen_pre RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX6849019 AqStaminode_pre RNA-Seq of Stage 11 Aquilegia staminode SRP221616 bp0 Total RNA was isolated using the Qiagen RNeasy Plant Mini kit (Qiagen, Valencia, CA, USA), including a repeated elution step. RNA quality was analyzed using a NanoDrop 8000 spectrophotometer (Thermofisher) and an Agilent Bioanalyzer. RNA samples with a minimum 2.09 260/280, and RNA integrity number of 7.5 were used as input using the Apollo Prep X PolyA8 mRNA 200 bp beta V1 protocol for single-end library generation. All libraries were quality confirmed for correct size distributions by TapeStation, quantified by QBIT and quantitative PCR using the ABI prism 7900 and Kappa quantification kit and pooled before running on one Illumina HiSeq2500 lane. SRS5387480 Staminode Pre AqStaminode_pre RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX6849020 AqStamen_post RNA-Seq of Stage 13 Aquilegia stamen SRP221616 bp0 Total RNA was isolated using the Qiagen RNeasy Plant Mini kit (Qiagen, Valencia, CA, USA), including a repeated elution step. RNA quality was analyzed using a NanoDrop 8000 spectrophotometer (Thermofisher) and an Agilent Bioanalyzer. RNA samples with a minimum 2.09 260/280, and RNA integrity number of 7.5 were used as input using the Apollo Prep X PolyA8 mRNA 200 bp beta V1 protocol for single-end library generation. All libraries were quality confirmed for correct size distributions by TapeStation, quantified by QBIT and quantitative PCR using the ABI prism 7900 and Kappa quantification kit and pooled before running on one Illumina HiSeq2500 lane. SRS5387481 Stamen Post AqStamen_post RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500 SRX6849021 AqStaminode_post RNA-Seq of Stage 13 Aquilegia staminode SRP221616 bp0 Total RNA was isolated using the Qiagen RNeasy Plant Mini kit (Qiagen, Valencia, CA, USA), including a repeated elution step. RNA quality was analyzed using a NanoDrop 8000 spectrophotometer (Thermofisher) and an Agilent Bioanalyzer. RNA samples with a minimum 2.09 260/280, and RNA integrity number of 7.5 were used as input using the Apollo Prep X PolyA8 mRNA 200 bp beta V1 protocol for single-end library generation. All libraries were quality confirmed for correct size distributions by TapeStation, quantified by QBIT and quantitative PCR using the ABI prism 7900 and Kappa quantification kit and pooled before running on one Illumina HiSeq2500 lane. SRS5387482 Staminode Post AqStaminode_post RNA-Seq TRANSCRIPTOMIC cDNA Illumina HiSeq 2500