SRX6885070 GSM4088932 SRP222892 SRS5418311 GSM4088932 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088932 GSM4088932 GEO Accession GSM4088932 SRX6885071 GSM4088933 SRP222892 SRS5418312 GSM4088933 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088933 GSM4088933 GEO Accession GSM4088933 SRX6885072 GSM4088934 SRP222892 SRS5418313 GSM4088934 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088934 GSM4088934 GEO Accession GSM4088934 SRX6885073 GSM4088935 SRP222892 SRS5418314 GSM4088935 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088935 GSM4088935 GEO Accession GSM4088935 SRX6885074 GSM4088936 SRP222892 SRS5418315 GSM4088936 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088936 GSM4088936 GEO Accession GSM4088936 SRX6885075 GSM4088937 SRP222892 SRS5418316 GSM4088937 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088937 GSM4088937 GEO Accession GSM4088937 SRX6885076 GSM4088938 SRP222892 SRS5418317 GSM4088938 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088938 GSM4088938 GEO Accession GSM4088938 SRX6885077 GSM4088939 SRP222892 SRS5418318 GSM4088939 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088939 GSM4088939 GEO Accession GSM4088939 SRX6885078 GSM4088940 SRP222892 SRS5418319 GSM4088940 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088940 GSM4088940 GEO Accession GSM4088940 SRX6885079 GSM4088941 SRP222892 SRS5418320 GSM4088941 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088941 GSM4088941 GEO Accession GSM4088941 SRX6885080 GSM4088942 SRP222892 SRS5418321 GSM4088942 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088942 GSM4088942 GEO Accession GSM4088942 SRX6885081 GSM4088943 SRP222892 SRS5418322 GSM4088943 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088943 GSM4088943 GEO Accession GSM4088943 SRX6885082 GSM4088944 SRP222892 SRS5418323 GSM4088944 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088944 GSM4088944 GEO Accession GSM4088944 SRX6885083 GSM4088945 SRP222892 SRS5418324 GSM4088945 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088945 GSM4088945 GEO Accession GSM4088945 SRX6885084 GSM4088946 SRP222892 SRS5418325 GSM4088946 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088946 GSM4088946 GEO Accession GSM4088946 SRX6885085 GSM4088947 SRP222892 SRS5418326 GSM4088947 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088947 GSM4088947 GEO Accession GSM4088947 SRX6885086 GSM4088948 SRP222892 SRS5418327 GSM4088948 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088948 GSM4088948 GEO Accession GSM4088948 SRX6885087 GSM4088949 SRP222892 SRS5418328 GSM4088949 RNA-Seq TRANSCRIPTOMIC cDNA Aortas and carotid arteries from male and female Apoe-/- Cx3cr1GFP Cd11cYFP fed WD for 5 months were enzymatically digested. Single cell suspensions were stained with CD45 and 7AAD. Live, CD45+ cells were flow sorted into GFP+ YFP- (GFP), GFP+YFP+ (DP), GFP-YFP+(YFP), and GFP-YFP- (DN) groups using a FACSAria II directly into Trizol LS. RNA was extracted from the sorted GFP, DP, YFP, and DN cells using an RNeasy Mini Kit (Qiagen) following the standard protocol. Total RNA was processed using SMART-seq v4 Ultra Low Input RNA Kit (Clonetech) following manufacturer's instructions. cDNA was amplified using 11 cycles and eluted in 12 μL. 5 μL of resulting cDNA was processed using a NexteraXT kit (Illumina) following manufacturer's instructions. Illumina HiSeq 4000 gds 304088949 GSM4088949 GEO Accession GSM4088949