SRX7070392 GSM4143932 SRP227389 SRS5586660 GSM4143932 miRNA-Seq TRANSCRIPTOMIC size fractionation Whole colonic or jejunal GI tissue was extracted and dispersed for cellular dissociation and prepared for cell sorting. Utilizing Fluoresence Activated Cell Sorting (FACS), cells positive for GFP signal were isolated as these cells represent Kit+ in GI tissue, which are ICC. These isolated KitcopGFP/+ cells were then placed into Lysis/Binding Buffer from the mirVana miRNA isolation kit (Thermo-Fisher) and extracted under manufacturer protocol. Samples from same genotypes (15 each) were pooled together to create the sample ready for processing and sequencing. miRNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 304143932 GSM4143932 GEO Accession GSM4143932 SRX7070393 GSM4143933 SRP227389 SRS5586661 GSM4143933 miRNA-Seq TRANSCRIPTOMIC size fractionation Whole colonic or jejunal GI tissue was extracted and dispersed for cellular dissociation and prepared for cell sorting. Utilizing Fluoresence Activated Cell Sorting (FACS), cells positive for GFP signal were isolated as these cells represent Kit+ in GI tissue, which are ICC. These isolated KitcopGFP/+ cells were then placed into Lysis/Binding Buffer from the mirVana miRNA isolation kit (Thermo-Fisher) and extracted under manufacturer protocol. Samples from same genotypes (15 each) were pooled together to create the sample ready for processing and sequencing. miRNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 304143933 GSM4143933 GEO Accession GSM4143933 SRX7070394 GSM4143934 SRP227389 SRS5586662 GSM4143934 miRNA-Seq TRANSCRIPTOMIC size fractionation Whole colonic or jejunal GI tissue was extracted and dispersed for cellular dissociation and prepared for cell sorting. Utilizing Fluoresence Activated Cell Sorting (FACS), cells positive for GFP signal were isolated as these cells represent Kit+ in GI tissue, which are ICC. These isolated KitcopGFP/+ cells were then placed into Lysis/Binding Buffer from the mirVana miRNA isolation kit (Thermo-Fisher) and extracted under manufacturer protocol. Samples from same genotypes (15 each) were pooled together to create the sample ready for processing and sequencing. miRNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 304143934 GSM4143934 GEO Accession GSM4143934 SRX7070395 GSM4143935 SRP227389 SRS5586663 GSM4143935 miRNA-Seq TRANSCRIPTOMIC size fractionation Whole colonic or jejunal GI tissue was extracted and dispersed for cellular dissociation and prepared for cell sorting. Utilizing Fluoresence Activated Cell Sorting (FACS), cells positive for GFP signal were isolated as these cells represent Kit+ in GI tissue, which are ICC. These isolated KitcopGFP/+ cells were then placed into Lysis/Binding Buffer from the mirVana miRNA isolation kit (Thermo-Fisher) and extracted under manufacturer protocol. Samples from same genotypes (15 each) were pooled together to create the sample ready for processing and sequencing. miRNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 304143935 GSM4143935 GEO Accession GSM4143935