SRX7184564 GSM4178164 SRP230631 SRS5690788 GSM4178164 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178164 GSM4178164 GEO Accession GSM4178164 SRX7184565 GSM4178165 SRP230631 SRS5690789 GSM4178165 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178165 GSM4178165 GEO Accession GSM4178165 SRX7184566 GSM4178166 SRP230631 SRS5690790 GSM4178166 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178166 GSM4178166 GEO Accession GSM4178166 SRX7184567 GSM4178167 SRP230631 SRS5690791 GSM4178167 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178167 GSM4178167 GEO Accession GSM4178167 SRX7184568 GSM4178168 SRP230631 SRS5690792 GSM4178168 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178168 GSM4178168 GEO Accession GSM4178168 SRX7184569 GSM4178169 SRP230631 SRS5690793 GSM4178169 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178169 GSM4178169 GEO Accession GSM4178169 SRX7184570 GSM4178170 SRP230631 SRS5690794 GSM4178170 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178170 GSM4178170 GEO Accession GSM4178170 SRX7184571 GSM4178171 SRP230631 SRS5690795 GSM4178171 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178171 GSM4178171 GEO Accession GSM4178171 SRX7184572 GSM4178172 SRP230631 SRS5690796 GSM4178172 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178172 GSM4178172 GEO Accession GSM4178172 SRX7184573 GSM4178173 SRP230631 SRS5690797 GSM4178173 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178173 GSM4178173 GEO Accession GSM4178173 SRX7184574 GSM4178174 SRP230631 SRS5690798 GSM4178174 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178174 GSM4178174 GEO Accession GSM4178174 SRX7184575 GSM4178175 SRP230631 SRS5690799 GSM4178175 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178175 GSM4178175 GEO Accession GSM4178175 SRX7184576 GSM4178176 SRP230631 SRS5690800 GSM4178176 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178176 GSM4178176 GEO Accession GSM4178176 SRX7184577 GSM4178177 SRP230631 SRS5690801 GSM4178177 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178177 GSM4178177 GEO Accession GSM4178177 SRX7184578 GSM4178178 SRP230631 SRS5690802 GSM4178178 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178178 GSM4178178 GEO Accession GSM4178178 SRX7184579 GSM4178179 SRP230631 SRS5690803 GSM4178179 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178179 GSM4178179 GEO Accession GSM4178179 SRX7184580 GSM4178180 SRP230631 SRS5690804 GSM4178180 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178180 GSM4178180 GEO Accession GSM4178180 SRX7184581 GSM4178181 SRP230631 SRS5690805 GSM4178181 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178181 GSM4178181 GEO Accession GSM4178181 SRX7184582 GSM4178182 SRP230631 SRS5690806 GSM4178182 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178182 GSM4178182 GEO Accession GSM4178182 SRX7184583 GSM4178183 SRP230631 SRS5690807 GSM4178183 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178183 GSM4178183 GEO Accession GSM4178183 SRX7184584 GSM4178184 SRP230631 SRS5690808 GSM4178184 RNA-Seq TRANSCRIPTOMIC cDNA After 3 h, cells were centrifuged and cell pellet was lysed in 700 μL Trizol (Thermo-Fisher) for RNA preparation. A time zero sample was also collected and lysed in Trizol. Three independent experiments were completed and analyzed separately. RNA was purified using the Direct-Zol Mini Prep RNA Isolation Kit (Zymo R2052) with DNaseI on-column treatment. Approximately 500ng of each total RNA was converted into a sequencing library using the TruSeq mRNA Strand Specific Library Prep kit from Illumina, and 1x150 base single end reads were collected on an Illumina HiSeq V4/4000 instrument at the University of Colorado Genomics Core. Each total RNA sample (7 experimental conditions; 3 biological replicates) generated library was individually bar-coded, all the libraries were mixed and sequence data collected over 3 lanes. Illumina HiSeq 4000 gds 304178184 GSM4178184 GEO Accession GSM4178184