SRX7191470 GSM4182505 SRP230786 SRS5697485 GSM4182505 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182505 GSM4182505 GEO Accession GSM4182505 SRX7191471 GSM4182506 SRP230786 SRS5697486 GSM4182506 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182506 GSM4182506 GEO Accession GSM4182506 SRX7191472 GSM4182507 SRP230786 SRS5697487 GSM4182507 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182507 GSM4182507 GEO Accession GSM4182507 SRX7191473 GSM4182508 SRP230786 SRS5697488 GSM4182508 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182508 GSM4182508 GEO Accession GSM4182508 SRX7191474 GSM4182509 SRP230786 SRS5697489 GSM4182509 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182509 GSM4182509 GEO Accession GSM4182509 SRX7191475 GSM4182510 SRP230786 SRS5697490 GSM4182510 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182510 GSM4182510 GEO Accession GSM4182510 SRX7191476 GSM4182511 SRP230786 SRS5697491 GSM4182511 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182511 GSM4182511 GEO Accession GSM4182511 SRX7191477 GSM4182512 SRP230786 SRS5697492 GSM4182512 ChIP-Seq GENOMIC ChIP Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182512 GSM4182512 GEO Accession GSM4182512 SRX7191478 GSM4182513 SRP230786 SRS5697493 GSM4182513 ATAC-seq GENOMIC other Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182513 GSM4182513 GEO Accession GSM4182513 SRX7191479 GSM4182514 SRP230786 SRS5697494 GSM4182514 ATAC-seq GENOMIC other Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182514 GSM4182514 GEO Accession GSM4182514 SRX7191480 GSM4182515 SRP230786 SRS5697495 GSM4182515 ATAC-seq GENOMIC other Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182515 GSM4182515 GEO Accession GSM4182515 SRX7191481 GSM4182516 SRP230786 SRS5697496 GSM4182516 ATAC-seq GENOMIC other Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304182516 GSM4182516 GEO Accession GSM4182516 SRX7196648 GSM4183295 SRP230786 PRJNA590698 SRS5702209 SAMN13340412 ATAC-seq GENOMIC other Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. For ChIP-seq, anti-Opa antibodies were raised in two rabbits (E990 and E991) against a truncated recombinant protein spanning from amino acids 125-507 of Opa. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304183295 GSM4183295 GEO Accession GSM4183295 SRX7196649 GSM4183296 SRP230786 PRJNA590698 SRS5702210 SAMN13340411 ATAC-seq GENOMIC other Embryos produced from either wild-type (D. melanogaster.ZH-86Fb) or mutant sh opa and sh zld were collected on yeasted apple juice agar plates. Individual embryos were selected from plates , and nuclear morphology was observed under a compount microscope at 20x magnification. Temperatures for sample collection were maintained between 22°C and 24°C to minimize variation in cell cycle timing. Under these conditions, cell cycle times were indistinguishable. Cell cycles were timed from the onset of anaphase of the previous cell cycle. The staging of samples at 3-min intervals relies on the synchrony of nuclear divisions in the early Drosophila embryos. Each embryo was hand-selected and hand-dechorionated for the analysis. For ChIP-seq, anti-Opa antibodies were raised in two rabbits (E990 and E991) against a truncated recombinant protein spanning from amino acids 125-507 of Opa. Fragmentation and amplification of ATAC-seq libraries were performed essentially as described previously (Buenrostro et al., 2015). Prepared libraries were subject to single-end sequencing using an Illumina HiSeq2500. Illumina HiSeq 2500 gds 304183296 GSM4183296 GEO Accession GSM4183296