SRX7191853 GSM4182922 SRP230797 SRS5697868 GSM4182922 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182922 GSM4182922 GEO Accession GSM4182922 SRX7191855 GSM4182923 SRP230797 SRS5697870 GSM4182923 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182923 GSM4182923 GEO Accession GSM4182923 SRX7191856 GSM4182924 SRP230797 SRS5697871 GSM4182924 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182924 GSM4182924 GEO Accession GSM4182924 SRX7191857 GSM4182925 SRP230797 SRS5697872 GSM4182925 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182925 GSM4182925 GEO Accession GSM4182925 SRX7191858 GSM4182926 SRP230797 SRS5697873 GSM4182926 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182926 GSM4182926 GEO Accession GSM4182926 SRX7191859 GSM4182927 SRP230797 SRS5697874 GSM4182927 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182927 GSM4182927 GEO Accession GSM4182927 SRX7191860 GSM4182928 SRP230797 SRS5697875 GSM4182928 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182928 GSM4182928 GEO Accession GSM4182928 SRX7191861 GSM4182929 SRP230797 SRS5697876 GSM4182929 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182929 GSM4182929 GEO Accession GSM4182929 SRX7191862 GSM4182930 SRP230797 SRS5697877 GSM4182930 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182930 GSM4182930 GEO Accession GSM4182930 SRX7191863 GSM4182931 SRP230797 SRS5697878 GSM4182931 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted from mouse spleen cells using the RNeasy Mini Kit (Qiagen) and following manufacturer's guidelines. Cells were homogenized with QIAshredder colums (Qiagen). DNA digestion was performed using RNase-free DNase (Qiagen). RNA integrity was assessed using the LabChip GXII Touch HT electrophoresis system, with the RNA Assay and the DNA 5K / RNA / Charge Variant Assay LabChip (all PerkinElmer). RNA samples were stored at -70°C. RNA sequencing method was designed based on the Drop-seq protocol described in (Macosko EZ et al. Cell 2015). Briefly, 10 ng of RNA was mixed with Indexing Oligonucleotides. After 5 minutes of incubation the RNA was combined with RT mix (1 x Maxima RT buffer, 1 mM dNTPs, 10 U/µl Maxima H- RTase, 1 U/µl RNase inhibitor and 2.5 µM Template Switch Oligo). Samples were incubated for 30 minutes at 22°C and 90 minutes at 42°C. The constructed cDNA was amplified by PCR. The PCR products were pooled together in sets of 12 samples containing different Indexing Oligos and purified with Agencourt AMPure XP Beads according to the manufacturer's instructions. The 3'-end cDNA fragments for sequencing were prepared using the Nextera XT (Illumina) tagmentation reaction. The reaction was performed according to manufacturer's instruction, with the exception of the P5 SMART primer that was used instead of S5xx Nextera primer. Each set of 12 samples that was pooled after the PCR reaction was tagmented with a different Nextera N7xx index. Subsequently, the samples were PCR amplified and purified twice using Agencourt AMPure Beads. The libraries were sequenced on a Illumina NextSeq 500, with a custom primer producing read 1 of 20 bp and read 2 (paired end) of 50 bp. Sequencing was performed at the Functional Genomics Unit of the University of Helsinki, Finland. NextSeq 500 gds 304182931 GSM4182931 GEO Accession GSM4182931