Comment[GEAAccession]	E-GEAD-287
MAGE-TAB Version	1.1
Investigation Title	Whole axon profiling with iPSCs-derived motor neurons comparing Control and FUS-ALS.
Experiment Description	iPSCs-derived motor neurons (MNs) were cultured on microfluidic device for 20 days. Then, axon was devided from somato-dendrites (SDs) and RNA was purified from each other. Whole RNA profiling with axon and SDs from control and FUS-ALS mutant MNs were analyzed with RNA-sequencing.
Experimental Design	cell type comparison design	disease state design	genetic modification design
Experimental Factor Name	genotype] Factor Value[tissue
Experimental Factor Type	genotype] Factor Value[tissue
Person Last Name	Akiyama
Person First Name	Tetsuya
Person Affiliation	ALS, Neurology, Tohoku University Graduate School of Medicine
Person Roles	submitter
Public Release Date	2022-11-01
Protocol Name	P-GEAD-44	P-GEAD-45	P-GEAD-46	P-GEAD-47	P-GEAD-48
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	iPSCs-derived motor neurons (MNs) were cultured on microfluidic device for 20 days. Then, axon was devided from somato-dendrites (SDs) and RNA was purified from each other. Whole RNA profiling with axon and SDs from control and FUS-ALS mutant MNs were analyzed with RNA-sequencing.	Total RNA was purified with RNeasy micro kit.	A total of 12 ng of axonal total RNA and 1 microg of SD total RNA with the use of a SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) and a TruSeq RNA stranded mRNA Sample Prep Kit (Illumina), respectively.	Libraries were clonally amplified on a flow cell and sequenced on a HiSeq2500 (HiSeq Control Software v2.2.58, Illumina) with a 51-mer single-end sequence.	Cufflinks (ver. 2.2.1) was used to estimate the gene expression level on the basis of fragments per kilobase of the exon model per million mapped fragments (FPKM) (Trapnell et al., 2013).
SDRF File	E-GEAD-287.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB7603
Comment[Last Update Date]	2022-11-01