Comment[GEAAccession]	E-GEAD-288
MAGE-TAB Version	1.1
Investigation Title	Gene expression in mouse intestines with Strongyloides venezuelensis infection
Experiment Description	To identify genes differentially expressed in worm-infected (Strongyloides venezuelesnsis) mice relative to naive mice. Gene expression of mouse intestine was analysed using GeneChip Mouse Gene 1.0 ST Array.
Experimental Design	stimulus or stress design
Experimental Factor Name	treatment
Experimental Factor Type	treatment
Person Last Name	Kikuchi
Person First Name	Taisei
Person Affiliation	Parasitology, Faculty of Medicine, University of Miyazaki
Person Roles	submitter
Public Release Date	2022-11-06
Protocol Name	P-GEAD-49	P-GEAD-50	P-GEAD-51	P-GEAD-52	P-GEAD-53	P-GEAD-54
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol
Protocol Description	Mice were euthanised on 8th DPI and the anterior part of the duodenum (5-15 cm from the pylorus) of the small intestine was isolated and washed three times with PBS.	RNA concentration and quality were evaluated by Experion Automated Electrophoresis System (BioRad). Only RNA (100 ng) having RIN >7.5 was used for the microarray analysis.	RNA labelling, microarray hybridisation and scanning were performed using Ambion WT Expression Kit (Thermo Fisher Scientific) and GeneChip Mouse Gene 1.0 ST Array (Affimetrix, Santa Clara, CA, USA) with GeneChip Scanner 3000 (Affimetrix, Santa Clara, CA, USA), according to the manufacturer's instructions.	RNA labelling, microarray hybridisation and scanning were performed using Ambion WT Expression Kit (Thermo Fisher Scientific) and GeneChip Mouse Gene 1.0 ST Array (Affimetrix, Santa Clara, CA, USA) with GeneChip Scanner 3000 (Affimetrix, Santa Clara, CA, USA), according to the manufacturer's instructions.	RNA labelling, microarray hybridisation and scanning were performed using Ambion WT Expression Kit (Thermo Fisher Scientific) and GeneChip Mouse Gene 1.0 ST Array (Affimetrix, Santa Clara, CA, USA) with GeneChip Scanner 3000 (Affimetrix, Santa Clara, CA, USA), according to the manufacturer's instructions.	Normalisation and probe set summarisation were performed on each sample separately using the expression Transcriptome Analysis Console Software v4.0 (Affimetrix).
SDRF File	E-GEAD-288.sdrf.txt
Comment[Number of channel]	single-channel
Comment[Array Design REF]	A-AFFY-130
Comment[AEExperimentType]	transcription profiling by array
Comment[BioProject]	PRJDB7625
Comment[Last Update Date]	2022-11-06