Comment[GEAAccession]	E-GEAD-292
MAGE-TAB Version	1.1
Investigation Title	Conformation of the expression of neo-antigens in mouse cell lines
Experiment Description	"\"\"\"\"\"\"To determine the immunogenicity of mouse tumor, we performed an epitope discovery study.
RNA sequencing were used for conformation of identified candidate epitope.\"\"\"\"\"\""
Experimental Design	strain or line design
Experimental Factor Name	strain
Experimental Factor Type	strain
Person Last Name	Muraoka
Person First Name	Daisuke
Person Affiliation	Oncology, Nagasaki University
Person Roles	submitter
Public Release Date	2022-12-14
Protocol Name	P-GEAD-72	P-GEAD-73	P-GEAD-74	P-GEAD-75	P-GEAD-76
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	cell line	Total RNA was extracted with Qiagen RNeasy Kit	RNA-seq libraries were generated from RNA samples.	RNA sequencing was performed by Hokkaido System Science Co., Ltd. on a HiSeq 2000, resulting in 30 million RNA fragments from each library.	We aligned the RNA-seq reads to the mm9 genome with Tophat using the Bowtie aligner.
SDRF File	E-GEAD-292.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB7818
Comment[Last Update Date]	2022-12-14