Comment[GEAAccession]	E-GEAD-295
MAGE-TAB Version	1.1
Investigation Title	Transcriptome analysis of the stick insect Entoria okinawaensis
Experiment Description	RNA sequencing of midgut transcriptome of Entoria okinawaensis which is the biggest phasmid in Japan.
Experimental Design	replicate design
Experimental Factor Name	replicate
Experimental Factor Type	replicate
Person Last Name	Bono
Person First Name	Hidemasa
Person Affiliation	Database Center for Life Science, Research Organization of Information and Systems
Person Roles	submitter
Public Release Date	2021-05-06
Protocol Name	P-GEAD-87	P-GEAD-88	P-GEAD-89	P-GEAD-90	P-GEAD-91
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol
Protocol Description	Insect was cultured at room temperature in the laboratory.	RNA was extracted using PureLink RNA extraction kit (Thermo Fisher Scientific Inc., Valencia, CA, USA)	TruSeq RNA Sample Prep Kit v2 (Illumina) was used for library preparation, according to the manufacturer's protocol.	Illumina platform was used for the sequencing	After transcriptome assembly by Trinity, TPM values were calculated using kallisto.
SDRF File	E-GEAD-295.sdrf.txt
Comment[AEExperimentType]	RNA-seq of coding RNA
Comment[BioProject]	PRJDB7316
Comment[Last Update Date]	2021-05-06