Comment[GEAAccession]	E-GEAD-297
MAGE-TAB Version	1.1
Investigation Title	Comparison of gene expression profiles between IGFBP-2-knockdown and control glioblastoma cell line U251
Experiment Description	To examine the role of IGFBP-2 in gliuoblastoma (GBM) cell function, we used short hairpin RNA (shRNA) directed against IGFBP-2 mRNA. We constructed retroviral vectors which showed stable and significant silencing effects on IGFBP-2 genes of two different human GBM cell lines, U251 and YKG-1. Expression profiles of IGFBP-2-knockdown U251 with those from control cells are shown.
Experimental Design	genetic modification design
Experimental Factor Name	genetic modification
Experimental Factor Type	genetic modification
Person Last Name	Fukushima	Kataoka	Tezuka	Shimomura
Person First Name	Tsuyoshi	Hiroaki	Tomoaki	Takeshi
Person Affiliation	Pharmaceuticals Research Unit,Discovery Technology Laboratory I,Mitsubishi Pharma Corporation			
Person Roles	submitter	submitter	submitter	submitter
Public Release Date	2019-02-01
PubMed ID	17475624
Protocol Name	P-GEAD-100	P-GEAD-101	P-GEAD-102	P-GEAD-103	P-GEAD-104	P-GEAD-105	P-GEAD-106
Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid labeling protocol	nucleic acid hybridization to array protocol	array scanning and feature extraction protocol	normalization data transformation protocol	growth protocol
Protocol Description	Cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) at 37 degree_C in a humidified atmosphere containing 5% CO2.	Total RNAs were extracted with TrizolTM reagent (Gibco BRL, Gaithersburg, MD), followed by DNase I (Roche Applied Science, Indianapolis, IN) treatment and phenol-chloroform-isoamylalcohol extraction. The total RNA was reverse-transcribed with the T7-oligo(dT)24 primer followed by 2nd strand cDNA synthesis using a Super Script Choice System Kit (Invitrogen). The converted cDNA was repurified by phenol-chloroform-isoamyl alcohol and quality checked by electrophoresis.	One-cycle target labeling kit (Affymetrix)	Affymetrix standard procedure	By using GeneChip Scanner 3000 (Affymetrix) By using GCOS software (Affymetrix)	Scaling all probe sets for 100 (GCOS packaging)	cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) at 37degree_C in a humidified atmosphere containing 5% CO2.
SDRF File	E-GEAD-297.sdrf.txt
Comment[Number of channel]	single-channel
Comment[CIBEX Submitter]	Tsuyoshi Fukushima, Hiroaki Kataoka, Tomoaki Tezuka, Takeshi Shimomura
Comment[Array Design REF]	A-AFFY-113
Comment[AEExperimentType]	transcription profiling by array
Comment[SecondaryAccession]	CBX17
Comment[BioProject]	PRJDB7970
Comment[CIBEX Accept Date]	2007-05-07
Comment[CIBEX Public Release Date]	2007-05-07
Comment[Last Update Date]	2019-08-14